五味子醇乙促进小鼠肝增大的脂质组学研究  

Lipidomics analysis on schisandrol B-induced liver enlargement in mice

作　　者：赵颖媛 姚欣鹏 焦廷颖 田佳宁 周艳莹 高悦 陈盼盼 范仕成 黄民 毕惠嫦 ZHAO Ying-yuan;YAO Xin-peng;JIAO Ting-ying;TIAN Jia-ning;ZHOU Yan-ying;GAO Yue;CHEN Pan-pan;FAN Shi-cheng;HUANG Min;BI Hui-chang(Lab of Drug Metabolism and Pharmacokinetics,School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China)

机构地区：[1]中山大学药学院药物代谢与药动学实验室,广东广州510006

出　　处：《药学学报》2020年第5期922-929,共8页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81973392)。

摘　　要：五味子醇乙(schisandrol B, SolB)是传统中药五味子的活性单体之一。前期研究发现SolB在抵抗药源性肝损伤和促进肝再生的过程中,具有促进良性肝增大的作用,但肝增大过程中内源物质的变化尚不清楚。本研究通过脂质组学研究SolB所致肝增大过程的脂质组变化。本动物实验经中山大学动物伦理委员会批准。雄性C57BL/6小鼠腹腔注射SolB (100 mg·kg^-1·d^-1) 5 d后采集血清和肝脏样本进行生化和病理检测,采用基于Q Exactive UHPLCMS/MS系统的脂质组学方法对肝脏样本进行脂质组学检测与分析。结果发现SolB显著促进正常小鼠的良性肝增大(与肝损伤、炎症无关),小鼠肝脏出现脂质累积。脂质组学分析发现35种有显著差异的脂质,其中甘油三酯(triglycerides, TG)变化最为显著,其含量显著升高,提示SolB所致肝增大过程能量合成增加。本研究阐明了SolB所致肝增大的脂质组变化,初步解释了SolB促进肝增大过程对脂质的影响。Schisandrol B(SolB) is one of the active constituents from a traditional Chinese medicine Schisandra chinensis or Schisandra sphenanthera. Our previous studies found that SolB exerts hepatoprotective effects against drug-induced liver injury and promotes liver regeneration. We further found that SolB significantly induces liver enlargement but the mechanisms remain unclear. The purpose of this study was to investigate the change of lipidome in liver tissues during SolB-induced hepatomegaly. The animal experiment protocol was approved by the Institutional Animal Care and Use Committee at Sun Yat-sen University. Serum and liver samples of male C57 BL/6 mice were collected after intraperitoneal injection of SolB(100 mg·kg^-1·d^-1) for 5 days. Lipidomics analysis was performed using Q Exactive UHPLC-MS/MS system. The results showed that SolB significantly promoted liver enlargement in mice without liver injury and inflammation. Lipid accumulation was observed in the liver tissues after SolB treatment. Thirty-five lipids were identified with significant change and triglycerides(TG) were found to have the most significant increase in SolB-treated group, indicating the increase of energy production during SolB-induced hepatomegaly. This study reveals the impact of SolB on lipid metabolism and provides a potential explanation for liver enlargement induced by SolB.

关 键 词：五味子醇乙 肝增大 脂质组学 

分 类 号：R963[医药卫生—微生物与生化药学]