白茅内生菌Chaetomium globosum WQ产生的一个新细胞松弛素化合物  

A new cytochalasan compound from endophyte Chaetomium globosum WQ in Imperata cylindrical

作　　者：夏文静 曹兴琴 刘琴[3] 王莎莎 申丽 XIA Wen-jing;CAO Xing-qin;LIU Qin;WANG Sha-sha;SHEN Li(Institute of Translational Medicine,Medical College,Yangzhou University,Yangzhou 225001,China;Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases,Yangzhou 225001,China;Jiangsu Lixiahe Institute of Agricultural Science,Yangzhou 225007,China)

机构地区：[1]扬州大学医学院(转化医学研究院),江苏扬州225001 [2]江苏省中西医结合老年病防治重点实验室,江苏扬州225001 [3]江苏里下河地区农业科学研究所,江苏扬州225007

出　　处：《药学学报》2020年第5期967-970,共4页Acta Pharmaceutica Sinica

基　　金：江苏省高等学校自然科学研究重大项目(17KJA350005);江苏省重点研究计划(现代农业)项目(BE2017339);扬州市重点研发计划项目(YZ2017043,YZ2018063);扬州大学科技创新培育基金项目(2015CXJ074);江苏省研究生科研与实践创新计划项目(SJCX18_0807).

摘　　要：白茅内生菌Chaetomium globosum WQ固体发酵能产生丰富的细胞松弛素化合物。在前期实验基础上,以1H NMR谱和TLC为指导,采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和高效液相色谱等方法从C. globosum WQ固体发酵产物乙酸乙酯相浸膏中分离得到1个新细胞松弛素化合物,其结构经高分辨质谱、一维和二维核磁共振谱等波谱技术鉴定为20-iso-chaetoglobosin E (1)。Chaetomium globosum WQ, an endophyte derived from Imperata cylindrical, can produce abundant cytochalasan compounds through solid state fermentation. Based on previous research and guided by1 H NMR spectrum and TLC, a new cytochalasan compound was isolated from the ethyl acetate extract of a solid culture of C. globosum WQ using silica gel column chromatography, gel filtration over Sephadex LH-20 and HPLC. The new compound was characterized as 20-iso-chaetoglobosin E(1) by a combination of spectroscopic(HR-MS, 1D and2D NMR) analyses.

关 键 词：白茅 内生菌 Chaetomium globosum 次生代谢产物 20-iso-chaetoglobosin E 细胞松弛素化合物 

分 类 号：R284.2[医药卫生—中药学]