5株绿僵菌对橙带蓝尺蛾幼虫致病力比较  

Comparison of Virulence of Different Metarhizium anisopliae Strains Against Milionia basalis Larvae

作　　者：何学友[1] 蔡守平[1] 郑宏 曾丽琼[1] 陈文玉 林曦碧 詹祖仁 HE Xueyou;CAI Shouping;ZHENG Hong;ZENG Liqiong;CHEN Wenyu;LIN Xibi;ZHAN Zuren(Fujian Academy of Forestry,The Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China,Fuzhou 350012,Fujian,China;Forestry Diseases and Pests Control Station of Xinluo District,Longyan City,Longyan 364000,Fujian,China;Management Office of Quanzhou Forest Park,Quanzhou 362000,Fujian,China;Forestry Diseases and Pests Control Station of Mingxi County,Mingxi 365200,Fujian,China;Forestry Diseases and Pests Control Station of Youxi County,Youxi 365100,Fujian,China)

机构地区：[1]福建省林业科学研究院、国家林业局南方山地用材林培育重点实验室,福建福州350012 [2]龙岩市新罗区森林病虫害防治检疫站,福建龙岩364000 [3]泉州森林公园管理处,福建泉州362000 [4]明溪县森林病虫害防治检疫站,福建明溪365200 [5]尤溪县森林病虫害防治检疫站,福建尤溪365100

出　　处：《福建林业科技》2020年第2期15-19,共5页Journal of Fujian Forestry Science and Technology

基　　金：福建省林业科技推广项目(2019TG11);福建省森林培育与林产品加工利用重点实验室。

摘　　要：橙带蓝尺蛾(Milionia basalis)是近年来严重危害罗汉松和竹柏的一种重要食叶害虫,为筛选出对该虫具有高致病力的优良菌株,对5株不同来源的金龟子绿僵菌(Metarhizium anisopliae)(MaFZ-13、MaFZ-16、MaQL-02、MaXJ-04、MaZPTR-01)在PDA培养基上的培养性状及其对橙带蓝尺蛾幼虫的致病力进行测定。结果表明:不同绿僵菌菌株菌落的生长速度、产孢量及其对橙带蓝尺蛾幼虫的致病力差异十分显著。菌株接种培养15 d,菌落直径最大的为MaZPTR-01菌株,达6.5 cm;其次为MaFZ-13菌株,达6.2 cm。产孢量最高的为MaFZ-13,达1.33×10^8孢子·cm-2,MaZPTR-01菌株次之,达1.28×10^8孢子·cm-2。MaFZ-13、MaXJ-04菌株对橙带蓝尺蛾幼虫的致死率较高,15 d后分别达到96.7%、95.0%,其它菌株均低于90%;幼虫僵虫率分别为75%、71.7%;致死中时(LT50)也显著短于其它菌株,分别为5.01 d、5.48 d。绿僵菌MaFZ-13和MaXJ-04菌株不仅生长速度快、产孢量高,而且对橙带蓝尺蛾幼虫的致病力强,可作为优良菌株进一步开发利用。Milionia basalis is a new insect pest caused serious damage to Podocarpus macrophyllus and Podocarpus nagi in recent years.Biological characteristics on PDA media of 5 Metarhizium anisopliae strains(MaFZ-13,MaFZ-16,MaQL-02,MaXJ-04,MaZPTR-01)and their virulence against M.basalis larvae are tested for screening the virulent strains for this pest.The results showed there was significant difference in colony diameter,sporulation and virulence against M.basalis larvae among different strains.15 days after cultured on plate medium,the biggest diameter of colony were MaZPTR-01 strain,which was 6.5 cm,then was MaFZ-13 strain,which was 6.2 cm.MaFZ-13 and MaZPTR-01 were the strains with most sporulation,that could reach 1.33×10^8 spores·cm-2and 1.28×10^8 spores·cm-2,respectively.The results of virulence test showed mortality of M.basalis larvae 15 days after inoculated with MaFZ-13 and MaXJ-04 reached 96.7%and 95.0%,respectively,while the mortality of other strains were less than 90%.Muscardine cadaver rates of larvae inoculated with MaFZ-13 and MaXJ-04 were 75%and 71.7%,respectively.The median lethal time(LT50)of MaFZ-13 and MaXJ-04 against M.basalis larvae were 5.01 days ans 5.48 days,significantly shorter than other strains.MaFZ-13 and MaXJ-04 has fast growth,higher sporulation and good virulence against M.basalis larvae,so these two strains could be used for the further development.

关 键 词：金龟子绿僵菌 橙带蓝尺蛾 致死中时(LT50) 僵虫率 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治] S763.38[农业科学—植物保护]