聚集诱导发光探针分子在荧光传感中的应用  

Application of AIE-active probes in fluorescence sensing

作　　者：杜宪超 王佳[1] 秦安军[1] 唐本忠[1,2] Xianchao Du;Jia Wang;Anjun Qin;Benzhong Tang(Center for Aggregation-Induced Emmision,Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates,State Key Laborartory of Luminescent Materials and Devices,South China University of Technology,Guangzhou 510640,China;Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction,Department of Chemistry,The Hong Kong University of Science&Technology,Hong Kong,China)

机构地区：[1]华南理工大学发光材料与器件国家重点实验室,广东省分子聚集发光重点实验室,聚集诱导发光研究中心,广州510640 [2]香港科技大学化学系,国家人体组织功能重建工程技术研究中心香港分中心,香港

出　　处：《科学通报》2020年第15期1428-1447,共20页Chinese Science Bulletin

基　　金：国家自然科学基金(21788102,21525417,51620105009);广东省自然科学基金(2016A030312002,2019B030301003);香港创新及科技基金(ITC-CNERC14S01)资助。

摘　　要：荧光传感作为化学传感领域中的一项重大技术,具有灵敏度高、选择性好和响应快等优点,但是传统有机发光分子在高浓度或者聚集状态下,容易发生荧光强度的降低或是完全消失,这在一定程度上不利于其在应用中发挥最佳效果.聚集诱导发光(AIE)概念的提出为解决聚集导致发光猝灭(ACQ)的难题提供了方案,实现了发光分子在聚集态下的高荧光量子产率.具有AIE特性的发光分子被用作荧光传感器不仅具有高亮度的荧光信号,而且不必担心由于分子聚集导致的荧光信号的降低或猝灭.同时,由于某些分子聚集程度的增强导致的荧光颜色和强度的变化,可以被用来实现对靶标物的定性和定量分析.本文简述了近几年来AIE分子在荧光传感方面的应用,如离子检测、气体、有机小分子、爆炸物、蛋白质及酶等化学/生物传感器,同时对基于AIE分子的荧光传感器在设计和应用前景做了展望.Fluorescent probes that exhibit distinct advantages of high sensitivity, good selectivity, fast response speed, etc., have been widely applied in diverse sensing areas. However, the fluorescence of traditional organic probes tends to decrease or be quenched at high concentration or in aggregate state due to the aggregation-caused quenching(ACQ) effect, which limits their application in sensing areas. In 2001, our group coined the concept of aggregation-induced luminescence(AIE),which is exactly opposite to the ACQ effect and refers to a unique phenomenon that a kind of non-or weakly emissive luminogens in dilute solutions are induced to emit intensely upon aggregation or in solid state. Thus, the AIE-active luminogens(AIEgens) provide an alternative to solve this difficulty.Thanks to their intrinsic advantages, the fluorescent sensors based on AIEgens show lower background, higher signal-tonoise ratio and more outstanding resistance to photobleaching etc. Moroever, the AIE probes not only could overcome the problem encountered by ACQ ones, but also achieve faster and more sensitive detection of the targets. Thus, using AIE probes to detect a wide varity of targets is one of the hot research areas. To highlight the progress in this area, herein, we briefly summarize the sensing applications of AIEgens.First, we introduced the AIE probes used in the detection of ions, including K+, Hg2+, Fe3+, CN-, Cl O-, PO43-, etc.Notably, the fluorescent probes based on AIEgen could realize the selective detection of various ions not only in the organic solutions, but also in aqueous solutions. Next, we discussed the detection of low mass molecules using AIE probes for they play a very important role in life activities and their content can reflect the health status and life information of an organism.The used low mass molecules include gas(NH3, CO2, H2 S), explosive(PA, TNT), biothiol(Gys, GSH), and ATP and so on.Third, we summarized the qualitative and quantitative sensing of biomacromolecules, such as DNA, proteins, enzyme, b

关 键 词：聚集诱导发光 荧光传感 离子 小分子 生物大分子 

分 类 号：O657.3[理学—分析化学] TP212.2[理学—化学]