表面增强拉曼光谱技术在肿瘤标志物检测中的研究进展  

Recent advances in surface-enhanced Raman spectroscopy for the detection of tumor markers

作　　者：刘厦[1,2] 霍亚鹏 康维钧 高志贤[2] Sha Liu;Yapeng Huo;Weijun Kang;Zhixian Gao(School of Public Health,Hebei Medical University,Shijia zhuang 050017,China;Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety,Institute of Environmental and Operational Medicine,Academy of Military Medical Science,Academy of Military Science,Tianjin 300050,China;School of Public Health,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]河北医科大学公共卫生学院,石家庄050017 [2]军事科学院军事医学研究院环境医学与作业医学研究所,天津市环境与食品安全风险监控技术重点实验室,天津300050 [3]兰州大学公共卫生学院,兰州730000

出　　处：《科学通报》2020年第15期1448-1462,共15页Chinese Science Bulletin

基　　金：国家重点研发计划(2017YFC1200903,2017YFC1601205);天津市自然科学基金(17JCZDJC34500)资助。

摘　　要：恶性肿瘤已严重威胁我国居民健康,而肿瘤标志物的灵敏、准确检测对于癌症的早期诊断、治疗及预后评价至关重要.因此,探究一种灵敏、准确、快速、无创的检测技术,具有十分重要的临床意义.表面增强拉曼光谱(SERS)是利用光与金、银等纳米结构材料相互作用产生很强的表面等离子激元共振效应,可显著增强吸附在纳米结构表面上分子的拉曼信号,以超灵敏获取样品自身或拉曼探针分子丰富的指纹图谱.该技术具有非侵入性和灵敏度高、选择性好、分析速度快、水干扰小等独特优势,使其在生命科学、临床检验等方面具有良好的应用前景,成为了一种极具潜力的生物检测技术.本文主要综述了近5年SERS技术在蛋白质类、酶类、核酸类、细胞类、组织类、气体类和其他类肿瘤标志物检测中的研究进展,分析了该技术在生物检测中亟待解决的问题与挑战,并对其未来的发展前景进行了展望.Sensitive and accurate detection of tumor markers is critically essential for the early clinical diagnosis, treatment and prognosis of cancers. Surface-enhanced Raman spectroscopy(SERS) utilizes plasmonic nanomaterials to generate a strong surface plasmon resonance(SPR) effect in the visible to near-infrared region, such as silver and gold nanostructures. The SPR effect can significantly enhance the Raman signals of adsorbed molecules on the surface of nanostructures, allowing to gain the sensitive fingerprint information for the analyte itself or the Raman reporter. Benefitted from the superior properties of high sensitivity, good selectivity, fast and noninvasive analysis, SERS has attracted increasing interest and become a very promising technique for biological analysis. In comparison with other bioanalytical techniques, such as enzyme-linked immunosorbent assay, fluorescence and electrochemical methods, SERS possesses the following advantages:(1) SERS has an ultrahigh sensitivity, even down to single molecule level;(2) SERS can provide the intrinsic molecular fingerprint information, and the bandwidths of SERS peaks are usually very narrow;(3) SERS is suitable for long-term monitoring, because of the resistance of photobleaching and photodegradation;(4) non-invasive detection can be achieved with a low amount of sample and no preparation;(5) the interference of water is very weak, so biological samples can conveniently be detected in aqueous solutions;(6) various SERS-active nanostructures can be designed for multiplex detection. In this review, we provide an overview of recent advances in SERS technology for the detection of different tumor markers, including proteins, enzymes, nucleic acids, cells, tissues, gases and others. Great successes have been achieved by utilizing SERS, which can facilitate the fabrication of assays for tumor biomarkers.However, SERS has not been widely applied in clinical bioanalysis and biodiagnosis in the past over 40 years. SERS-based bioassay involves complex interactions betw

关 键 词：表面增强拉曼光谱 肿瘤标志物 生物检测 细胞 

分 类 号：O657.37[理学—分析化学] R730.4[理学—化学]