含氯消毒剂对新型冠状病毒核酸破坏效果研究  

Study on the effect of chlorine disinfectants on the destruction of New coronavirus nucleic acid

作　　者：陈茂义[1] 胡婕[1] 毛春林[1] 石韬[1] 陈婷[1] 曾玉洁 李斌[1] 益琼[1] 李虎 王黎 CHEN Maoyi;HU Jie;MAO Chunlin;SHI Tao;CHEN Ting;ZENG Yujie;LI Bin;YI Qiong;LI Hu;WANG Li(Jingzhou Center for Disease Control and Prevention,Jingzhou,Hubei 434000,China)

机构地区：[1]湖北省荆州市疾病预防控制中心,湖北荆州434000

出　　处：《公共卫生与预防医学》2020年第3期22-24,共3页Journal of Public Health and Preventive Medicine

摘　　要：目的观察含氯消毒剂对新型冠状病毒核酸的破坏效果,评估实时荧光定量PCR技术用于新型冠状病毒消毒效果评价的可行性。方法采用悬液定量灭活试验法,观察不同浓度含氯消毒剂、不同作用时间对新型冠状病毒的灭活效果,使用荧光定量PCR方法检测新型冠状病毒核酸进行消毒效果评价。结果1000 mg/L和2000 mg/L有效氯消毒剂作用30 s以上就能破坏新型冠状病毒核酸。750 mg/L有效氯消毒剂作用15 min以上能破坏新型冠状病毒核酸。500 mg/L有效氯消毒剂作用30 min不能完全破坏新型冠状病毒核酸。结论国家防控方案规定的使用1000 mg/L有效氯对新型冠状病毒疫点作用30 min的消毒方法,可以完全破坏新型冠状病毒核酸。可以使用实时荧光定量PCR技术用于新型冠状病毒消毒效果评价。Objective To observe the destructive effects of chlorine disinfectants on the nucleic acid of novel coronavirus,and to assess the feasibility of real-time fluorescent quantitative PCR technology for the evaluation of the disinfection effect of novel coronavirus.Methods A suspension quantitative inactivation test was used to observe the inactivation effects of different concentrations of chlorine containing disinfectants and different action times on the novel coronavirus.RT-PCR method was used to detect novel coronavirus nucleic acid for the disinfection effect evaluation.Results The chlorine disinfectants of 1000 mg/L and 2000 mg/L could destroy the nucleic acid of ew coronavirusafter application for over 30 s.The chlorine disinfectant of 750 mg/L could destroy the nucleic acid of new coronavirusafter application for over 15 min.Chlorine disinfectant of 500 mg/L could not completely destroy the nucleic acid of new coronavirusafter 30 min action.Conclusion The disinfection method stipulated in the national prevention and control plan using 1000 mg/L effective chlorine for the epidemic sites of new coronavirus for 30 minutes could completely destroy the nucleic acid of new coronavirus.The Real-time quantitative PCR can be used to evaluate the disinfection effect of new coronavirus.

关 键 词：含氯消毒剂 灭活效果 新型冠状病毒 荧光定量PCR 

分 类 号：R183[医药卫生—流行病学]