单宁酸对生长猪胃-小肠仿生消化中消化酶活性及饲粮粗蛋白消化率的影响  

Effects of Tannic Acid on Digestive Enzyme Activity and Digestibility of Dietary Crude Protein in Simulated Gastric and Intestinal Digestion for Growing Pigs

作　　者：王亚[1] 赵峰[1] 张虎[1] 赵威[2] 徐二华[2] 李浙烽[2] WANG Ya;ZHAO Feng;ZHANG Hu;ZHAO Wei;XU Erhua;LI Zhefeng(State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Provincial Key Agricultural Enterprise Research Institute of Encapsulated Feed Additive,King Techina Technology Co.,Ltd.,Hangzhou 311107,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193 [2]杭州康德权饲料有限公司包膜饲料添加剂省级重点农业企业研究院,杭州311107

出　　处：《畜牧兽医学报》2020年第6期1332-1341,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：中国农业科学院科技创新工程(ASTIP-IAS07);中国农业科学院北京畜牧兽医研究所-杭州康德权饲料有限公司合作项目(2017-YF-05)。

摘　　要：本试验旨在探讨单宁酸对生长猪胃、小肠仿生消化中消化酶活性及玉米-豆粕型饲粮干物质和粗蛋白消化率的影响,为评价单宁酸的生物学效应提供参考。试验一采用单因素完全随机设计,考察在无饲粮下2种单宁酸对猪模拟胃液、模拟小肠液消化酶活性的影响。设5个处理,单宁酸添加量分别为0 mg(胃液体积为20 mL,小肠液体积为22 mL);单宁酸1,10 mg;单宁酸1,20 mg;单宁酸2,10 mg;单宁酸2,20 mg。测定各处理的胃蛋白酶、淀粉酶、胰蛋白酶和糜蛋白酶的活性。试验二考察玉米-豆粕型饲粮添加单宁酸对猪仿生消化中胃、小肠阶段消化酶活性及养分消化率的影响。采用单因素完全随机设计,设5个处理,单宁酸在饲粮中的含量分别为0 mg·(2 g)^-1;单宁酸1,10 mg·(2 g)^-1;单宁酸1,20 mg·(2 g)^-1;单宁酸2,10 mg·(2 g)^-1;单宁酸2,20 mg·(2 g)^-1。测定仿生消化中胃阶段0.5和4 h时胃蛋白酶活性,小肠阶段0.5、4和8 h时淀粉酶、胰蛋白酶和糜蛋白酶活性及生长猪胃-小肠仿生消化测定饲粮的干物质和粗蛋白消化率。结果表明:1)无饲粮的情况下,和空白对照组相比,2种单宁酸对模拟胃液中胃蛋白酶活性无显著影响(P>0.05),单宁酸1比单宁酸2更高地降低了模拟小肠液中淀粉酶、胰蛋白酶和糜蛋白酶的活性(P<0.05)。2)在饲粮进行仿生消化的胃消化0.5~4 h内,除4 h时10 mg·(2 g)^-1添加量外,添加单宁酸1时胃蛋白酶的活性均显著高于添加单宁酸2时的相应值(P<0.05),除单宁酸2在消化0.5 h外,2种单宁酸在添加10 mg·(2 g)^-1时胃蛋白酶活性均显著高于20 mg·(2 g)^-1添加量的相应值(P<0.05)。在小肠仿生消化0.5 h时,饲粮中添加单宁酸1、2的2个水平对消化液中淀粉酶活性无显著影响(P>0.05),但均显著降低了糜蛋白酶的活性(P<0.05);单宁酸1的消化液中胰蛋白酶活性高于单宁酸2的相应值(P<0.05)。在小肠仿生消化4 h时,除添加水平为20 mgThis experiment was conducted to investigate the effects of tannic acid on digestive enzyme activity and digestibility of dry matter and crude protein of corn-soybean meal diet in the simulated gastric and small intestinal digestion for growing pigs,which will provide a reference for evaluating the biological effects of tannic acid.In the experiment 1,a single factor completely randomized arrangement was used to investigate the effects of 2 tannic acids without diet on digestive enzyme activities in simulated gastric and small intestinal fluid for pigs.Five treatments consisted of 0 mg tannic acid(the gastric fluid volume was 20 mL,the intestinal fluid volume was 22 mL);tannic acid 1,10 mg;tannic acid 1,20 mg;tannic acid 2,10 mg;tannic acid 2,20 mg.The activities of pepsin,amylase,trypsin and chymotrypsin were determined.The experiment 2 was to investigate the effects of tannic acid supplemention in corn-soybean meal diet on digestive enzyme activity in gastric and small intestinal simulated digestion phases and nutrient digestibility determined with the simulated digestion for growing pigs.Five treatments of dietary tannic acid concentration were 0 mg·(2 g)^-1;tannic acid 1,10 mg·(2 g)^-1;tannic acid 1,20 mg·(2 g)^-1;tannic acid 2,10 mg·(2 g)^-1;tannic acid 2,20 mg·(2 g)^-1,respectively.The activities of pepsin were measured at 0.5 and 4 h in the gastric digestion phase,and the activities of amylase,trypsin and chymotrypsin were measured at 0.5,4,and 8 h in the small intestinal digestion phase.Simultaneously,the digestibility of dry matter and crude protein were determined for the diet.The results showed that:1)Without the diet,compared with the blank control group,the 2 tannic acids didn’t significantly affect the activity of pepsin in the simulated gastric fluid(P>0.05);tannic acid 1 significantly reduced the activities of amylase,trypsin and chymotrypsin in the simulated intestinal fluid contrast to tannic acid 2(P<0.05).2)From 0.5 to 4 h of the simulated gastric digestion,except for the 10 mg·(2 g)^-

关 键 词：生长猪 仿生消化 单宁酸 消化酶活性 养分消化率 

分 类 号：S828[农业科学—畜牧学]