貉源阿留申病毒VP2和NS1蛋白的抗原性预测  

Cloning and Epitopes Analysis of VP2 and NS1 Proteins of Raccoon Dog and Arctic Fox Amdoparvovirus

作　　者：吴艳虹 赵永强 韦韬 章秀婷[1] 丛丽[1] 岳志刚[1] 肖家美[1] 邵西群[1] WU Yanhong;ZHAO Yongqiang;WEI Tao;ZHANG Xiuting;CONG Li;YUE Zhigang;XIAO Jiamei;SHAO Xiqun(Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《畜牧兽医学报》2020年第6期1399-1407,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：吉林省自然科学基金(20190201169JC);中国农业科学院农业科技创新工程(2019)。

摘　　要：旨在预测和分析貉源阿留申病毒(RFAV)VP2和NS1蛋白的抗原表位特征,筛选阿留申病毒属较保守的B、T细胞抗原表位。本研究对RFAV的近全长基因组进行克隆及测序,对其VP2和NS1基因编码蛋白的理化性质、二级结构、翻译后修饰位点和抗原表位进行预测,并将预测的修饰位点、抗原表位与其他阿留申病毒种的序列进行比较分析,筛选相对保守的修饰位点和抗原表位。结果显示,获得的RFAV基因组长4327 bp,编码VP2蛋白的636个氨基酸,编码NS1蛋白的641个氨基酸。VP2和NS1蛋白均为亲水性蛋白,二级结构以无规则卷曲为主。在阿留申病毒属内,VP2蛋白有3个保守的B细胞抗原表位,3个保守的T细胞表位,8个保守的翻译后修饰位点;NS1蛋白有1个保守的B细胞抗原表位,2个保守的T细胞抗原表位和7个保守的翻译后修饰位点。本研究成功克隆了RFAV近全长基因组序列,全面对RFAV的VP2、NS1蛋白抗原表位、翻译后修饰位点进行预测,并分析其在阿留申病毒属内的保守和变异特征,为阿留申病毒免疫研究提供参考。This study aimed to analyze the epitope characteristics of VP2 and NS1 proteins of raccoon dog and arctic fox amdoparvovirus(RFAV),and further select the conserved B-and T-cell epitopes on the VP2 and NS1 proteins of RFAV in Amdoparvoviru s genus.In the present study,the RFAV genome except the 5′and 3′end of the genome was cloned and sequenced,then the bioinformatics programs were used to predict the physical and chemical properties,Secondary structure,post-translational modifications and potential B-and T-cell epitopes of VP2 and NS1 proteins,and further compared the potential epitopes and post-translational modifications with the typical members within Amdoparvovirus genus.The 4327 bp RFAV genomic sequence in length,encoding 636 amino acids of VP2 protein and 641 amino acids of NS1 protein,was obtained.Both VP2 and NS1 proteins are hydrophilic proteins and the secondary structure were mainly composed of random coils.We predicted there were 3 conserved B-cell epitopes,3 conserved T-cell epitopes and 8 conserved post-translational modification sites on VP2 protein within the Amdoparvovirus genus;while NS1 protein had 1,2 and 7 in the respective conserved items.In this study,we cloned the main genome sequence of RFAV,and predicted the potential epitopes and post-translational modifications on VP2 and NS1 proteins of RFAV.This study provides fundamental basis for the development of a candidate vaccine and disease diagnosis of amdoparvoviruses.

关 键 词：阿留申病毒 VP2蛋白 NS1蛋白 抗原表位 翻译后修饰位点 

分 类 号：S852.659.2[农业科学—基础兽医学]