禽网状内皮组织增生病病毒感染对SPF雏鸡血液和局部淋巴组织CD4^+/CD8^+细胞及相关细胞因子表达的影响  

Effects of Avian Reticuloendotheliosis Virus Infection on the CD4^+/CD8^+Ratio and the Expression of Related Cytokines in SPF Chicks

作　　者：刘丹华 郑世民[1] 刘晓静 吕晓萍[1] 高雪丽[1] 刘超男[1] LIU Danhua;ZHENG Shimin;LIU Xiaojing;L Xiaoping;GAO Xueli;LIU Chaonan(Heilongjiang Key Laboratory of Experimental Animal and Comparative Medicine,College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China;JOINN Laboratories(Beijing),INC,Beijing 100176,China)

机构地区：[1]东北农业大学动物医学学院黑龙江省实验动物与比较医学重点实验室,哈尔滨150030 [2]北京昭衍新药研究中心股份有限公司,北京100176

出　　处：《畜牧兽医学报》2020年第6期1447-1454,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31472169)。

摘　　要：旨在探讨禽网状内皮组织增生病病毒(reticuloendotheliosis virus,REV)对SPF雏鸡血液和局部淋巴组织中T淋巴细胞数量以及相关细胞因子表达的影响。将96只1日龄SPF雏鸡随机分为REV感染组和对照组,应用流式细胞术、酸性α-醋酸萘酯酶染色(acidα-naphthyl acetate esterase,ANAE)、荧光定量PCR等方法对上述指标进行检测。试验数据表明,与对照组相比,感染组雏鸡血液CD4^+T淋巴细胞数量在第7-35天显著降低、CD8^+T淋巴细胞数量在第7-28天显著升高,CD4^+/CD8^+比值在第7-28天显著降低(均P<0.05或P<0.01);局部淋巴组织哈德尔腺(Hader’s gland,HG)、派伊尔结(Peyer’s patch,PP)和盲肠扁桃体(caecal tonsil,CT)中ANAE^+T淋巴细胞数量均显著降低(均P<0.05或P<0.01);GH、PP和CT中细胞因子IL-2、IFN-γ和TNF-α转录量都有不同程度升高。本研究表明,REV感染引起雏鸡血液中CD4^+T淋巴细胞数量降低、CD4^+/CD8^+T淋巴细胞比例失衡、局部淋巴组织中T淋巴细胞数量相对减少及细胞因子TNF-α转录持续升高与REV造成感染雏鸡细胞免疫功能显著降低密切相关。The study aimed to investigate the effects of avian reticuloendotheliosis virus(REV)on the number of T lymphocytes in blood and the expression of related cytokines in local lymphoid tissues of SPF chicks.Ninety-six 1-day-old SPF chicks were randomly divided into REV-infected group and control group.The above indexes were detected by Flow Cytometry,acidα-naphthyl acetate esterase(ANAE)and Quantitative Real-time PCR.The results showed that compared with the control group,the number of CD4^+T lymphocytes in the infected group was significantly decreased at day 7-35,and the number of CD8^+T lymphocytes was significantly increased at day 7-28,the CD4^+/CD8^+ratio was significantly decreased at day 7-28(all P<0.05 or P<0.01);the number of ANAE^+T lymphocytes in the local lymphoid tissues of Hader’s gland(HG),Peyer’s patch(PP)and caecal tonsil(CT)were significantly decreased(all P<0.05 or P<0.01);the transcription levels of cytokines IL-2,IFN-γand TNF-αin HG,PP and CT were increased in various degrees.These data indicated that REV infection caused a decrease in the number of CD4^+T lymphocytes in the peripheral blood of chickens,an imbalance of the ratio of CD4^+/CD8^+T lymphocytes,a relative decrease in the number of T lymphocytes in local lymphoid tissues,and a continuous increase in the expression of cytokines TNF-αare closely related to the significantly reduced cellular immune function of infected chickens caused by REV.

关 键 词：禽网状内皮组织增生病病毒 局部淋巴组织 细胞免疫 

分 类 号：S858.315.3[农业科学—临床兽医学]