O型口蹄疫病毒抗体化学发光免疫分析检测方法的建立  

Establishment of Chemiluminescent Immunoassay for Detection of Type O FMDV Virus Antibody

作　　者：孙雨[1] 宋晓晖[1] 肖颖[2] 王睿男[1] 李秀梅 任雪建 李雪刚 魏巍[1] 杨林[1] 王传彬[1] Sun Yu;Song Xiaohui;Xiao Ying;Wang Ruinan;Li Xiumei;Ren Xuejian;Li Xuegang;Wei Wei;Yang Lin;Wang Chuanbin(China Animal Disease Control Center,Beijing 102600,China;Chongqing Animal Disease Control Center,Chongqing 400000,China;Luoyang Modern Biotechnology Research Institute Co.,Ltd.,Luoyang,Henan 471000,China;Dengfeng Animal Disease Control Center,Dengfeng,Henan 452470,China)

机构地区：[1]中国动物疫病预防控制中心,北京102600 [2]重庆市动物疫病预防控制中心,重庆400000 [3]洛阳现代生物技术研究院有限公司,河南洛阳471000 [4]登封市畜牧业服务中心,河南登封452470

出　　处：《中国草食动物科学》2020年第3期47-52,共6页China Herbivore Science

基　　金：国家重点计划研发项目(2016YFD0500901);现代农业人才支撑计划(2130106-024);跨境动物疫病防控机制建设(2130114-FK);农业技术实验示范与服务支持(净化)(2130106-039);现代农业产业技术体系建设北京市创新团队。

摘　　要：为建立动物血清中O型口蹄疫病毒抗体化学发光免疫分析检测方法,通过获得的O型口蹄疫病毒VP1融合蛋白与相关单克隆抗体,制备了O型口蹄疫病毒抗体竞争化学发光检测试剂盒。结果表明:该方法能够定量检测猪、牛、羊血清中的O型口蹄疫病毒VP1蛋白抗体,与其他偶蹄类动物病原无交叉反应,具有良好的重复性;对该方法的特异性指标、敏感性指标进行了验证,同国产O型口蹄疫抗体检测试剂盒进行了比较,阳性样品符合率92.62%,阴性样品符合率92.14%,总符合率92.45%;重复性试验组内与组间变异系数分别低于10%和15%。综上,该研究建立的O型口蹄疫病毒抗体化学发光免疫分析检测方法特异性高、敏感性强,操作简单、快速,具有较高的应用推广价值。To establish chemiluminescence immunoassay method for Type O FMD virus antibody in animal serum,a competitive chemiluminescence assay kit for Type O FMD virus antibody was established by combining the obtained Type O FMD virus VP1 fusion protein with relevant monoclonal antibodies.The results showed that this method could specifically and quantitatively detect the antibody of Type O FMD virus in the serum of pigs,cattle and sheep.This method had high sensitivity and specificity,no cross-reaction to other related pathogens.Coefficient of variation within and between groups was less than 10%,and it had good repeatability.Compared with the domestic antibody detection kit for Type O FMD,the coincidence rate of positive samples was 92.62%,the coincidence rate of negative samples was 92.14%,and the total coincidence rate was 92.45%.The coefficient of variation within and between groups was less than 10%.The chemiluminescence immunoassay method for Type O FMD virus antibody established in this study is characterized by high specificity,high sensitivity,simple operation,high application and promotion value.

关 键 词：O型口蹄疫病毒 化学发光 免疫分析 检测方法 

分 类 号：S851.347.1[农业科学—预防兽医学]