核呼吸因子1抑制高糖环境下成骨细胞的凋亡和促炎症细胞因子分泌  

Nuclear respiratory factor 1 inhibits cell apoptosis and promotes pro-inflammatory cytokines secretion of osteoblasts in hyperglycemia environment

作　　者：李瑞 石婷婷 王强 张永先 Li Rui;Shi Tingting;Wang Qiang;Zhang Yongxian(Department of Traumatic Orthopedic Surgery,The 960th Hospital of the PLA Joint Logistic Support Force,Ji’nan,250031,China)

机构地区：[1]中国人民解放军联勤保障部队第九六〇医院骨创伤外科,济南250031

出　　处：《解剖学杂志》2020年第3期190-193,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金面上项目(81972793)。

摘　　要：目的:探讨核呼吸因子1(NRF1)调控高糖环境下成骨细胞的凋亡和促炎症细胞因子的分泌。方法:用LipofectamineTM 2000将NRF1或空载体质粒转染hFOB 1.19成骨细胞,分别为NRF1组和空载体组。采用免疫印迹检测高糖处理后成骨细胞NRF1蛋白表达水平。采用流式细胞术检测高糖处理后各组细胞凋亡率,ELISA法检测培养基中肿瘤坏死因子α(TNF-α)、白细胞介素(IL)1-β(IL-1β)和IL-6的水平。结果:高糖处理后,成骨细胞NRF1表达明显低于空白对照组,NRF1组成骨细胞凋亡率明显低于空载体组。高糖处理后,过表达NRF1的成骨细胞分泌的促炎症细胞因子水平显著较低。结论:NRF1可抑制高糖导致的成骨细胞凋亡,其机制可能与抑制TNF-α、IL-1β和IL-6分泌有关。Objective:To investigate the effect and mechanism of nuclear respiratory factor 1(NRF1)on osteoblast apoptosis in diabetic osteopathy.Methods:Western blotting was performed to examine Nrf1 protein expression level of hFOB 1.19 human osteoblasts,which were treated with high glucose.The NRF1 or vector plasmid were transfected into osteoblasts hFOB 1.19 by LipofectamineTM 2000,and a blank control was also prepared for comparison.Highglucose treatment was administered to the infected cells.And then the cell apoptosis rate was examined with flow cytometry analysis.ELISA assays were also performed to detect the levels of tumor necrosis factor(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in the cultured medium of infected cells.Results:After highglucose treatment,the expression of NRF1 in osteoblasts was significantly lower than the control group.Overexpression of NRF1 in osteoblasts showed a significant low apoptosis rate with high-glucose treatment.After highglucose treatment,the level of pro-inflammatory factors secreted by osteoblasts overexpressing Nrf1 was significantly lower in the cultured medium.Conclusion:NRF1 can inhibit high glucose induced cell apoptosis of human osteoblasts.The underlying mechanism is correlated with the inhibition of TNF-α,IL-1βand IL-6 secretion.

关 键 词：糖尿病性骨病 成骨细胞 核呼吸因子1 凋亡 促炎症细胞因子 

分 类 号：R587.1[医药卫生—内分泌]