御唐丸对2型糖尿病大鼠空腹血清胰岛素、胰岛素抵抗指数、甘油三酯、总胆固醇水平及胰腺组织胰岛素表达的影响  

Effect of Yutang pill on fasting serum insulin,insulin resistance index,triglyceride,total cholesterol and insulin expression in pancreas of diabetic rats

作　　者：郑南 谢宁[1] 李全[2] 周妍妍[1] 高丽娟[3] ZHENG Nan;XIE Ning;LI Quan(School of Basic Medicine,Heilongjiang University of Traditional Chinese Medicine,Harbin,Heilongjiang 150040)

机构地区：[1]黑龙江中医药大学基础医学院,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [3]黑龙江中医药大学附属第一医院风湿科,黑龙江哈尔滨150040

出　　处：《河北中医》2020年第3期432-436,447,I0001,共7页Hebei Journal of Traditional Chinese Medicine

基　　金：黑龙江省2017年博士后资助项目(编号:LBH-Z17206);黑龙江中医药大学博士创新基金项目(编号:2017bs03);教育部春晖计划资助项目(编号:5030411)。

摘　　要：目的观察御唐丸对2型糖尿病大鼠空腹血清胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、甘油三酯(TG)、总胆固醇(TC)水平及胰腺组织胰岛素表达的影响。方法将150只大鼠随机分为空白对照组20只及造模组130只,造模组以链脲佐菌素(STZ)腹腔注射制作2型糖尿病模型,并选取造模成功的大鼠100只随机分为御唐丸高剂量组、御唐丸低剂量组、糖尿乐胶囊组、盐酸二甲双胍组及模型对照组,每组各20只。御唐丸高、低剂量组分别按2.70、1.35 g/(kg·d)予御唐丸药液灌胃,糖尿乐胶囊组按0.09 g/(kg·d)予糖尿乐胶囊药液灌胃,盐酸二甲双胍组按2.43 g/(kg·d)予盐酸二甲双胍片药液灌胃,空白对照组及模型对照组分别予2 mL/d蒸馏水灌胃,均连续灌胃8周。比较各组大鼠灌胃后血清FINS、HOMA-IR、TG、TC水平及胰腺组织胰岛素表达情况。结果与空白对照组比较,模型对照组灌胃后血清FINS、HOMA-IR、TC及TG水平均明显升高(P<0.05),胰腺组织胰岛素表达明显降低(P<0.05)。与模型对照组比较,糖尿乐胶囊组、盐酸二甲双胍组、御唐丸低剂量组及御唐丸高剂量组灌胃后血清FINS、HOMA-IR、TC及TG水平均明显下降(P<0.05),盐酸二甲双胍组、御唐丸低剂量组及御唐丸高剂量组胰腺组织胰岛素表达明显升高(P<0.05)。与糖尿乐胶囊组比较,御唐丸高剂量组灌胃后血清FINS、HOMA-IR、TC及TG水平均明显降低(P<0.05),胰腺组织胰岛素表达明显升高(P<0.05);御唐丸低剂量组灌胃后仅HOMA-IR及TG水平明显降低(P<0.05)。与盐酸二甲双胍组比较,御唐丸高剂量组灌胃后血清FINS水平明显降低(P<0.05),御唐丸低剂量组TC水平明显升高(P<0.05)。与御唐丸低剂量组比较,御唐丸高剂量组灌胃后血清FINS及TC水平均明显降低(P<0.05),胰腺组织胰岛素表达水平明显升高(P<0.05)。结论御唐丸对糖尿病大鼠有确切疗效,可明显降低大鼠血清FINS及HOMA-IR水平,并提高胰腺Objective To observe the effect of Yutang pill on fasting serum insulin(FINS),insulin resistance index(HOMA-IR),triglyceride(TG),total cholesterol(TC)and insulin expression in pancreas of diabetic rats.Methods 150 rats were randomly divided into blank control group(20 rats)and model group(130 rats).The diabetic model was made by intraperitoneal injection of streptozotocin(STZ)in model group,and the rats were randomly divided into three groups:Yutang pill high dose group,Yutang pill low dose group,Tangniaole capsule group,metformin hydrochloride group and model control group.The high and low dose groups of Yutang pills were given 2.70 and 1.35 g/(kg·d)Yutang pill liquid respectively by gavage.The Tangniaole capsule group were given 0.09 g/(kg·d)of Tangniaole capsule liquid by gavage.The metformin hydrochloride group was given 2.43 g/(kg·d)metformin hydrochloride liquid by gavage.The blank control group and model control group were given equal volume distilled water by gavage.All groups were treated for 8 weeks.The levels of FINS,HOMA-IR,TG,TC and the expression of insulin in pancreas were compared between each group.Results Compared with the blank control group,the levels of FINS,HOMA-IR,TC and TG in the model control group were significantly higher(P<0.05),and the expression of insulin in pancreatic tissue was significantly lower(P<0.05).Compared with the model control group,the levels of FINS,HOMA-IR,TC and TG were decreased significantly in the Tangniaole capsule group,metformin hydrochloride group,Yutang pill low dose group and Yutang pill high dose group(P<0.05).However,the expression of insulin in pancreas tissue of metformin hydrochloride group,Yutang pill low dose group and Yutang pill high dose group increased significantly(P<0.05).Compared with Tangniaole capsule group,the levels of FINS,HOMA-IR,TC and TG in high dose group were significantly lower than those in Tangniaole capsule group(P<0.05),and the expression of insulin in pancreatic tissue was significantly higher than that in Tangniaole capsule

关 键 词：糖尿病 2型 胰岛素 甘油三酯 胆固醇 大鼠 动物实验 中药疗法 

分 类 号：R285.5[医药卫生—中药学] R587.1[医药卫生—中医学]