感染白粉病烟株叶片真菌的群落结构与多样性  

Community Structure and Diversity of Fungi in Leaves of Tobacco Plant Infected with Powdery Mildew

作　　者：黄宇 汪汉成 陈乾丽 向立刚 郭珍妮[3] 李忠[1] 谢红炼 韩洁 HUANG Yu;WANG Hancheng;CHEN Qianli;XIANG Ligang;GUO Zhenni;LI Zhong;XIE Honglian;HAN Jie(College of Agriculture,Guizhou University,Guiyang,Guizhou 550025;Guizhou Academy of Tobacco Science,Guiyang,Guizhou 550081;College of Life Sciences,Yangtze University,Jingzhou,Hubei 434025;College of Agriculture,Yangtze University,Jingzhou,Hubei 434025;College of Basic Medical Science,Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550312,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]贵州省烟草科学研究院,贵州贵阳550081 [3]长江大学生命科学学院,湖北荆州434025 [4]长江大学农学院,湖北荆州434025 [5]贵州中医药大学基础医学院,贵州贵阳550312

出　　处：《贵州农业科学》2020年第5期54-58,I0001,共6页Guizhou Agricultural Sciences

基　　金：贵州省科技支撑计划项目“密集烘烤过程中烟叶腐烂病病害成因及其防控技术研究”[黔科合支撑(2018)2356];贵州省科技厅优秀青年人才培养计划项目“集约化育苗下烟草立枯病的流行规律及其防控技术研究”[黔科合平台人才(2017)5619];中国烟草总公司贵州省公司科技项目“互联网+烟草植保的研究与应用”(201714);中国烟草总公司科技项目“烟草主要叶斑类病害监测预警及绿色防控技术研究与应用”[110202001035(LS-04)];贵州省科学技术基金项目“三尖杉药用内生真菌诱变选育及其药理活性研究”[黔科合J字(2012)2065]。

摘　　要：为烟草白粉病的科学防治提供理论依据,采用Illumina MiSeq高通量测序技术对采自同一烟株上发病烟叶和健康烟叶DNA的ITS区进行测序,分析各样品的真菌群落结构与多样性。结果表明:白粉病烟株健康烟叶真菌群落丰富度及多样性均高于发病烟叶;2种样本的优势菌门均为子囊菌门(Ascomycota)和担子菌门(Basidiomycota);发病烟叶优势菌属及其相对丰富度为高氏白粉菌属(Golovinomyces)99.22%,曲霉属(Aspergillus)0.33%和链格孢属(Alternaria)0.33%;健康烟叶的优势菌属及其相对丰富度为高氏白粉菌属(Golovinomyces)91.86%、曲霉属(Aspergillus)0.34%和链格孢属(Alternaria)0.31%。在白粉病烟株上发病烟叶和健康烟叶均存在大量的高氏白粉菌属。The community structure and diversity of fungi in healthy and diseased tobacco leaves are analyzed by sequencing of ITS region of DNA in diseased and healthy leaves of the same tobacco plant through Illumina MiSeq high-throughput sequencing technique to provide the theoretical basis for scientific control of tobacco powdery mildew.Result:The community abundance and diversity of fungi in healthy leaves of tobacco plants infected with powdery mildew are higher than diseased leaves.The dominant phyla of two tested samples are Ascomycota and Basidiomycota.The dominant genera of diseased tobacco leaves are Golovinomyces,Aspergillus and Alternaria,and their relative abundance are 99.22%,0.33%and 0.33%respectively.The dominant genera of healthy tobacco leaves are Golovinomyces,Aspergillus and Alternaria,and their relative abundance are 91.86%,0.34%and 0.31%respectively.There is a large amount of Golovinomyces in diseased and healthy leaves of tobacco plants infected with powdery mildew.

关 键 词：烟草白粉病 Illumina高通量测序 真菌多样性 群落结构 

分 类 号：S572.435[农业科学—烟草工业]