可降解苯酚的厌氧产电菌株的分离筛选及生物学特性研究  

Isolation and Screening of a Strain of Anaerobic Electricity-producing Phenol Degrading Bacteria and Biological Characteristics Research

作　　者：李金达 易梦雯 王丽丽[1,2] 付春娜 燕红 LI Jin-da;YI Meng-wen;WANG Li-li;FU Chun-na;YAN Hong(School of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China;Key Laboratory of Green Chemical Technology of College of Heilongjiang Province, Harbin 150040, China)

机构地区：[1]哈尔滨理工大学化学与环境工程学院,哈尔滨150040 [2]黑龙江省高校绿色化工技术重点实验室,哈尔滨150040

出　　处：《哈尔滨理工大学学报》2020年第2期146-155,共10页Journal of Harbin University of Science and Technology

基　　金：国家自然科学基金(21776054)。

摘　　要：以含苯酚的厌氧段污水为底物构建微生物燃料电池(microbial fuel cell,MFC),从处于稳定期的MFC阳极分离筛选获得一株可降解苯酚的厌氧产电菌株A4。对菌株A4进行生理生化鉴定,表明该菌为肠杆菌。以菌株A4作为研究对象,探究了不同的氮源、碳源、pH值、温度、初始苯酚浓度、接种量及重金属离子对菌株生长同时对苯酚降解情况的影响。研究表明:最适氮源为NH4NO3,最佳共代谢基质为蔗糖,培养基初始pH值8,最佳初始苯酚浓度500 mg/L,最适温度为23℃,接种量为10%。菌株A4可耐受一定浓度(0.2 mg/L)的Pb2+,而对高浓度(2000 mg/L)的苯酚耐受性降低。该菌株在生长的稳定期产电,测得64 h时苯酚降解率达到67.37%,库伦效率(CE)为17.8%。研究通过选育优质的厌氧产电菌并优化其生长条件,在MFC处理含酚废水的应用上有重要意义。Microbial Fuel Cell(MFC)was constructed using phenol-containing anaerobic wastewater as a substrate.An anaerobic electrogenic strain A4 capable of degrading phenol was obtained by separating and screening the stable period microbial fuel cell anode.Physiological and biochemical identification of strain A4 showed that the strain was Enterobacter.Used strain A4 as the research object,the effects of different nitrogen sources,carbon sources,pH value,temperature,initial phenol concentration and heavy metal ion on the growth of strain A4 and phenol degradation were investigated.Studies have shown that NH4NO3 was the best nitrogen source,sucrose was the best co-metabolic substrate,the optimum pH value was 8,the optimal initial phenol concentration was 500 mg/L,the optimum temperature was 23℃,and the optimum inoculum was 10%.Strain A4 could tolerate a certain concentration(0.2 mg/L)of Pb2+,while this strain was less tolerant to high concentrations(2000 mg/L)of phenol.Strain A4 produced electricity during the stationary phase of growth.The phenol degradation rate reached 67.37%at 64 h and the coulombic efficiency(CE)was 17.8%.The research selected high-quality anaerobic electricity-producing bacteria and optimized their growth conditions,which is of great significance for the application of MFC in the treatment of phenol-containing wastewater.

关 键 词：苯酚降解 产电细菌 分离筛选 生物学特性 微生物燃料电池 

分 类 号：O643.3[理学—物理化学] Q939.9[理学—化学]