模型化合物诱导木质素降解菌株烟曲霉Aspergillus fumigatus G-13的产酶特性  

Study on Ligninase Activity of Aspergillus fumigatus G-13 Induced by Lignin Model Compounds

作　　者：冯茜 燕红[1,2] FENG Qian;YAN Hong(School of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040,China;Key Laboratory of Green Chemical Technology of College of Heilongjiang Province, Harbin 150040, China)

机构地区：[1]哈尔滨理工大学化学与环境工程学院,哈尔滨150040 [2]黑龙江省高校绿色化工技术重点实验室,哈尔滨150040

出　　处：《哈尔滨理工大学学报》2020年第2期156-165,共10页Journal of Harbin University of Science and Technology

基　　金：国家自然科学基金(21776054)。

摘　　要：以木质素酶活力作为指标,采用阿魏酸、对香豆酸和芥子酸3种模型化合物作为底物,通过添加纤维素及葡萄糖并改变培养方式,研究A.Fumigatus G-13诱导产酶特性。结果表明,摇床培养较静置培养菌株产酶能力提高了2~5倍;单独采用木质素模型化合物作为诱导物,芥子酸诱导作用最强,其LiP、Lac和MnP最大酶活分别可达24.08、21.67、110.78 U/L;羧甲基纤维素、微晶纤维素和葡萄糖作为共底物,均对3种模型化合物对菌株的诱导能力起促进作用,其中葡萄糖的促进作用最为明显(较原酶活提高约1.38~5.34倍),羧甲基纤维素的促进效果优于微晶纤维素;且纤维素模型化合物和葡萄糖在诱导产酶方面起协同作用。With the ligninase activity as an index,three model compounds,ferulic acid,p-coumaric acid and sinapic acid,were used as substrates to study the characteristics of Aspergillus fumigatus G-13 induced enzyme production by adding cellulose and glucose and changing the culture mode.The results showed that the ability of producing enzymes in shake flask culture was 2-5 times higher than that in static culture.The lignin model compound was used as inducer alone,and the induction effect of sinapic acid was the strongest.The maximum activities of LiP,Lac and MnP were 24.08,21.67 and 110.78 U/L,respectively.Carboxymethyl cellulose(CMC),microcrystalline cellulose(MCC)and glucose as co-substrates all promoted the induction ability of the three model compounds to the strain.Among them,glucose was the most prominent(about 1.38-5.34 times higher than the original enzyme activity),and CMC was better than microcrystalline cellulose.Cellulose model compounds and glucose played a synergistic role in the induction of enzyme production.

关 键 词：木质素模型化合物 诱导产酶 木质素酶 烟曲霉菌 

分 类 号：O643.3[理学—物理化学] Q939.9[理学—化学]