高效液相色谱法同时测定化妆品中7种植物美白活性成分  

Simultaneous Determination of Seven Kinds of Plant Whitening Active Ingredients in Cosmetics by High Performance Liquid Chromatography

作　　者：高家敏[1] 代静 李红霞[1] 段静 刘彤彤 曹进[1] 王钢力[1] GAO Jiamin;DAI Jing;LI Hongxia;DUAN Jing;LIU Tongtong;CAO Jin;WANG Gangli(National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《香料香精化妆品》2020年第3期61-65,共5页Flavour Fragrance Cosmetics

摘　　要：采用高效液相色谱法(HPLC)同时测定化妆品中7种植物美白活性成分。样品经质量分数70%甲醇溶液提取、过滤后,进行HPLC分析。色谱柱为Agilent ZORBAX Eclipse XDB-C18柱(4.6 mm×250 mm×5μm)。流动相组成为乙腈-质量分数0.05%磷酸溶液,梯度洗脱。流速为1.0 m L/min。二极管阵列检测器,红景天苷、甘草苷、丹皮酚、光甘草定的检测波长为275 nm,阿魏酸、芦荟苷、白藜芦醇的检测波长为300 nm。结果发现,7种植物美白活性成分均在2~100μg/m L范围内线性关系良好(相关系数r>0.999),霜类、乳液类、水剂类3种化妆品基质中7种植物美白活性成分的回收率(添加水平分别为质量分数0.004%、0.02%和0.04%)在86.8%~101.1%之间,相对标准偏差(RSD)在0.3%~4.8%范围内,最低检出浓度在质量分数0.0001%~0.0006%之间。A quantitative method for simultaneous determination of 7 kinds of plant whitening active ingredients in cosmetics by high performance liquid chromatography(HPLC)was established.Samples were extracted by mass fraction of 70%methanol solution,then separated on a Agilent ZORBAX Eclipse XDB-C18 column(4.6 mm×250 mm×5μm)with the mobile phase of acetonitrile and mass fraction of 0.05%phosphoric acid solution.The flow rate was 1.0 mL/min with gradient elution.Diode array detector(DAD)was used with the wavelength of 275 nm for salidroside,liquiritin、paeonol and glabridin,and 300 nm for ferulic、aloin and resveratrol.The calibration curves showed good linearity between 2 and 100μg/mL(r>0.999).The recoveries(spiked at mass fraction of 0.004%,0.02%and 0.04%,respectively)in 3 different types of cosmetic ranged from 86.8%to 101.1%,and the RSD range was 0.3%to 4.8%.The lower limit of detection of the 7 kinds of plant whitening active ingredients were in the range of mass fraction of 0.0001%to 0.0006%.

关 键 词：化妆品 植物美白活性成分 高效液相色谱法 含量测定 

分 类 号：O657.72[理学—分析化学] TQ658[理学—化学]