高压气腹对老龄小鼠腹腔探查手术术后认知功能的影响  

Effect of high pressure pneumoperitoneum on cognitive function in aged mice after abdominal exploration surgery

作　　者：胡慈贤 蒋婧妍 孟波 叶海旺 安秀君 李晓瑜 秦金玲 陈骏萍 卢波 HU Cixian;JIANG Jingyan;MENG Bo(Department of Anesthesiology,Yinzhou Hospital Affiliated to Medical School of Ningbo University,Ningbo 315040,China)

机构地区：[1]宁波市鄞州人民医院麻醉科 [2]中国科学院大学宁波华美医院(宁波市第二医院)麻醉科,315040 [3]不详

出　　处：《浙江医学》2020年第9期890-892,909,共4页Zhejiang Medical Journal

基　　金：浙江省自然科学基金(LQ19H090004);浙江省医药卫生科技计划项目(2019KY182、2018KY157);吴阶平医学基金会临床科研专项资助基金(320.6750.18191)。

摘　　要：目的观察高压气腹对老龄小鼠腹腔探查手术术后认知功能的影响。方法将64只12~14月龄小鼠随机分为正常组(N组)、手术组(S组)、手术+低压气腹组(L组,气腹压2mmHg)和手术+高压气腹组(H组,气腹压8mmHg),N组不进行干预,S组接受腹腔探查手术,L组和H组先进行气腹实验再进行腹腔探查手术。每组再分两个亚组(8只一组),分别于术后3d和7d进行行为学测试(包括新物体识别实验和条件性恐惧实验)。结果术后3d,S组、L组和H组的辨别指数(RI)均低于N组(均P<0.05);术后7d,L组和H组的RI均低于N组(均P<0.01)。术后3、7d,S组、L组和H组的场景诱发僵直时间均低于N组(均P<0.05),H组均低于L组(均P<0.05)。术后3、7d,S组、L组和H组的线索诱发僵直时间均低于N组(均P<0.05),H组均低于L组(均P<0.05)。结论相比低气腹压,高气腹压力会加剧手术麻醉所致老龄小鼠的恐惧记忆损害。Objective To investigate the effect of high-pressure pneumoperitoneum on cognitive function in aged mice after abdominal exploration surgery.Methods Sixty-four aged mice were randomly divided into 4 groups:group N received no treatment;group S received abdominal exploration surgery,and group L and group H underwent pneumoperitoneum experiment before abdominal exploration surgery,with pnermoperitoneum pressure of 2 mmHg and 8 mmHg,respectively.New object recognition test and fear conditioning test were used to evaluate cognitive function at postoperative day 3 and 7,respectively.Results Three days after surgery,the recognition index(RI)of group S,group L and group H were all shorter than that of N group(all P<0.05).Seven days after surgery,RI of group L and group H was shorter than that of groupN(all P<0.05).At 3 and 7 days after surgery,the time of context-induced freezing in group S,group L and group H was shorter than that in group N(all P<0.05);and the time of context-induced freezing in group H was shorter than that of group L(all P<0.05).At 3 and 7 days after surgery,the time of cue-induced freezing in group S,group L and group H was shorter than that in groupN(all P<0.05);and the time of cue-induced freezing in group H was shorter than that in group L(all P<0.05).Conclusion Compared with low-pressure pneumoperitoneum,high-pressure pneumoperitoneum aggravates the impairment of fear memory induced by surgery in aged mice.

关 键 词：气腹 术后认知功能 老龄 新物体识别 条件恐惧 

分 类 号：R614[医药卫生—麻醉学]