The antibacterial activity of Berberis heteropoda Schrenk and its effect on irritable bowel syndrome in rats  

作　　者：LI Li ZHU Hui-Min YAN Qi LI Song-Ya LI Fei 

机构地区：[1] College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China [2] State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing 210009,China

出　　处：《Chinese Journal of Natural Medicines》2020年第5期356-368,共13页中国天然药物（英文版）

基　　金：grants from the National Natural Science Foundation of China (Nos.81760645,81860773,81873185 and U1703108);Xinjiang Science Fund for Distinguished Young Scholar Project (No.2018Q003);Natural Science Foundation of Jiangsu province (No.BK20181327)。

摘　　要：The dried roots of Berberis heteropoda Schrenk have traditionally been used to treat acute gastroenteritis and dysentery. The aim of this study was to confirm the antibacterial activity of an extract of Berberis heteropoda Schrenk root in vitro and its therapeutic effects on rats with diarrhea-predominant irritable bowel syndrome(D-IBS) in vivo, as well as to identify the related signaling pathways. A water extract of Berberis heteropoda Schrenk root(BHS) inhibited the growth of S. aureus, E. coli, P. aeruginosa and S. faecalis. BHS potentially damaged the structure of the bacterial cell membrane and decreased the activity of some membranous enzymes, eventually killing the S. aureus, E. coli, P. aeruginosa and S. faecalis bacteria. Oral administration of BHS(low, middle and high dose group, L, M and H) significantly alleviated the abdominal pain, diarrhea, and depression-like symptoms of D-IBS rats, and the efficacy index ranged from 30% to 60%, indicating that the BHS treatment was effective. BHS(L, M and H) alleviated the abnormal pathological changes in the brain, as evidenced by HE staining. The expression of CHAT, 5-HT, C-FOS and CGRP was reduced by the BHS treatment(L, M and H). Our findings provide novel insights into the use of the natural product BHS to inhibit pathogenic bacteria by destroying the bacterial structure, indicating that BHS possesses certain biological activities. Furthermore, BHS has the potential to alleviate diarrhea, abdominal pain and depression-like behaviors in D-IBS rats by regulating the brain-gut peptide levels.

关 键 词：Diarrhea-type irritable bowel syndrome Antibacterial activity Antidiarrhea Anti-abdominal pain Antidepressant effects Berberis heteropoda Schrenk roots 

分 类 号：R965[医药卫生—药理学]