大气压低温等离子体处理对拟南芥幼苗抗氧化系统的影响  

Effect of cold atmospheric pressure plasmas treatment on antioxidant system in Arabidopsis thaliana seedlings

作　　者：王佳琪 崔东洁 阴悦 李亚霖 焦浈[1] WANG Jiaqi;CUI Dongjie;YIN Yue;LI Yalin;JIAO Zhen(Henan Key Laboratory of Ion-beam Bioengineering,College of Agricultural Sciences,Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]河南省离子束生物工程重点实验室郑州大学农学院,郑州450052

出　　处：《植物生理学报》2020年第3期423-430,共8页Plant Physiology Journal

基　　金：国家自然科学基金(11605159和11704343);中国博士后科学基金(2017M612412);河南省高校重点师资基金。

摘　　要：以拟南芥(Arabidopsis thaliana)幼苗为研究对象,探究大气压低温等离子体(CAPPs)对其活性氧(ROS)产生及抗氧化系统等变化的影响。采用植物生理学、分子生物学与细胞生物学相结合的方法,动态检测等离子体处理引发的拟南芥幼苗的抗氧化反应。结果表明等离子体处理促进拟南芥幼苗的生长,显著提高幼苗体内ROS、抗氧化酶和非酶类抗氧化物的积累,其中根尖ROS的含量变化最为明显。同时,呼吸爆发氧化酶同源基因(RBOHs)的表达水平也显著提高。综上所述,在等离子体处理促进拟南芥幼苗生长过程中,抗氧化系统起着重要作用。Reactive oxygen species(ROS) production and antioxidant system of Arabidopsis thaliana seedlings induced by cold atmospheric pressure plasmas(CAPPs) treatment were studied in this study. The combination of plant physiology, molecular biology and cell biology was used to dynamically investigate the antioxidant response of Arabidopsis seedlings induced by plasma treatment. Results showed that plasma treatment promoted the growth of Arabidopsis seedlings, and significantly increased the ROS accumulation, antioxidant enzymes activities and non-enzymatic antioxidants content in the seedlings. The change of ROS content at apical was more obvious. At the same time, the expression levels of respiratory burst oxidase homologs gene(RBOHs) were also markedly increased. In conclusion, antioxidant system plays an important role in the process of the stimulated growth of Arabidopsis induced by plasma treatment.

关 键 词：大气压低温等离子体 拟南芥 活性氧 抗氧化系统 

分 类 号：Q945[生物学—植物学]