基于2b-RAD技术的糯玉米品种‘沪玉糯3号’特异SNP分子标记开发  

Development of SNP markers for specific identification of waxy maize variety ‘Huyunuo3’ based on 2b-RAD technique

作　　者：卢媛[1,2] 韩晴 王义发[1] 施标 沈雪芳[1,2] LU Yuan;HAN Qing;WANG Yifa;SHI Biao;SHEN Xuefang(Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;CIMMYT-China Specialty Maize Research Center,Shanghai 201403,China)

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201403 [2]CIMMYT-中国特用玉米研究中心,上海201403

出　　处：《植物生理学报》2020年第3期573-582,共10页Plant Physiology Journal

基　　金：上海市种业发展项目[沪农科种字(2016)第1-3号];上海市科学技术委员会科研计划项目(153919N1300、17391900100和17391902702);上海市农业科学院卓越团队项目(农科创2017B-08);上海市市级农口系统青年人才成长计划[沪农青字(2015)第1-4号、沪农青字(2018)第1-6号];2019上海市农业科学院青年科技人员“助跑”计划。

摘　　要：利用2b-RAD (type IIB endonucleases restriction-site associated DNA)技术对糯玉米品种‘沪玉糯3号’及其亲本进行简化基因组测序。通过与玉米‘B73’参考基因组序列进行比对,在3个糯玉米样品的共同测序标签内筛选出20 498个单核苷酸多态性(single nucleotide polymorphism, SNP)位点,并基于RADtyping分型策略对它们进行基因型分型。对其中20 333个位于玉米10条染色体上的SNP位点的基因型进行分析,开发出6个可用于‘沪玉糯3号’品种特异性鉴定的SNP分子标记。Simplified genome sequencing technology was applied in the waxy maize variety ‘Huyunuo3’ and its parents by using 2 b-RAD(type IIB endonucleases restriction-site associated DNA) technology. Total 20 498 single nucleotide polymorphisms(SNPs) were detected in shared sequence tags of three samples by screening with maize ‘B73’ reference genome sequence and genotype calling with RADtyping strategy. Analyzing the genotypes of 20 333 SNPs that located on 10 chromosomes of maize, 6 SNP molecular markers were developed for the specific identification of ‘Huyunuo3’.

关 键 词：糯玉米‘沪玉糯3号’ 2b-RAD SNP 品种特异性 

分 类 号：S513[农业科学—作物学]