纳米金溶胶的绿色制备及其在三聚氰胺可视化检测中的应用  

Green preparation of gold nanoparticles hydrosols and its application in melamine visual detection

作　　者：周美双 李琼霞 付云芝[1] Meishuang;LI Qiongxia;FU Yunzhi(School of Science,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学理学院,海口570228

出　　处：《扬州大学学报（自然科学版）》2020年第2期8-14,共7页Journal of Yangzhou University：Natural Science Edition

基　　金：国家自然科学基金资助项目(21563009)。

摘　　要：海草提取液为反应介质,采用生物还原法成功制备酒红色纳米金溶胶(Au nanoparticles,AuNPs),并探究反应温度、提取液用量和提取液初始pH对制备AuNPs的影响.结果表明:实验最佳条件为50℃下,向1.00 mL初始pH为12.12的海草提取液中加入5.00 mL 1 mmol·L^-1氯金酸溶液磁力搅拌30 min.将AuNPs用于三聚氰胺(Melamine,Mel)的可视化检测,利用紫外-可见光谱进行验证,并通过傅里叶红外光谱、X射线光电子能谱和透射电子显微镜对AuNPs和检测样品进行表征与分析.根据检测结果可知,Mel标准溶液的浓度在0.4~0.8μmol·L^-1范围内呈线性相关,R^2=0.989,检出限为0.359μmol·L^-1.Using seaweed extract as the reaction medium,wine-red gold nanoparticles(Au nanoparticles,AuNPs)are successfully prepared by biological reduction method.The effects of reaction temperature,amount of extract and initial pH of extract on the preparation of AuNPs are investigated.The results show that the optimum condition of the experiment is to add 5.00 mL of 1 mmol·L^-1 chloroauric acid solution to 1.00 mL of seaweed extract with an initial pH of 12.12 at 50℃and stir magnetically for 30 min.By ultraviolet-visible spectroscopy,AuNPs are used for the visual detection of melamine(Melamine,Mel),and the AuNPs and the detected samples are characterized and analyzed by Fourier infrared spectroscopy,X-ray photoelectron spectroscopy,and transmission electron microscopy.According to the test results,when the concentration of Mel standard solution is 0.4~0.8μmol·L^-1,there is a linear correlation,R^2=0.989,and the detection limit is 0.359μmol·L^-1.

关 键 词：纳米金 三聚氰胺 紫外吸收光谱 可视化检测 

分 类 号：O657.3[理学—分析化学] TQ420.7[理学—化学]