改良型富血小板纤维蛋白膜包裹颗粒软骨移植的长期组织学改变  

Long-term histological changes of diced cartilage grafts wrapped in advanced platelet-rich fibrin membrane

作　　者：杨银辉 杨帆 樊旭 徐风瑞 郑梁 刘薇 何明武 Yang Yinhui;Yang Fan;Fan Xu;Xu Fengrui;Zheng Liang;Liu Wei;He Mingwu(Department of Plastic Surgery and Burns,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,Hubei Province,China;Basic Medical College,Hubei University of Medicine,Shiyan 442000,Hubei Province,China)

机构地区：[1]湖北医药学院附属医院(十堰市太和医院)整形美容烧伤科,湖北省十堰市442000 [2]湖北医药学院基础医学院,湖北省十堰市442000

出　　处：《中国组织工程研究》2020年第34期5486-5492,共7页Chinese Journal of Tissue Engineering Research

基　　金：太和医院科研经费(14Y22),项目负责人:何明武。

摘　　要：背景:已有学者研究并制备了改良型富血小板纤维蛋白,并通过动物实验证实了改良型富血小板纤维蛋白可以促进自体软硬组织的再生与修复。目的:制备改良型富血小板纤维蛋白膜,观察改良型富血小板纤维蛋白膜及自体筋膜包裹颗粒软骨移植术后移植物的组织学改变。方法:新西兰雄兔12只,均进行以下相同手术流程:制备改良型富血小板纤维蛋白膜,取兔左耳软骨组织及右后肢自体筋膜组织,制备成3种移植物:颗粒软骨;自体筋膜包裹颗粒软骨;改良型富血小板纤维蛋白膜包裹颗粒软骨。将移植物分别回植于兔背部皮下,术后4个月取出移植物,进行一般形态观察和组织病理学检测。结果与结论:①自体筋膜和改良型富血小板纤维蛋白膜包裹颗粒软骨移植物厚度较颗粒软骨移植物厚,移植物外形较圆润,表面较平整;②改良型富血小板纤维蛋白膜包裹颗粒软骨移植物软骨细胞存活率和再生潜力明显优于另外2种移植物(P <0.05);其他组织学参数各组之间无显著差异;③结果说明:改良型富血小板纤维蛋白膜包裹颗粒软骨移植能够提高颗粒软骨存活率和移植物塑形效果,减少移植后软骨再吸收。BACKGROUND:Advanced platelet-rich fibrin has been studied and prepared by scholars.Advanced platelet-rich fibrin has been proven to promote the regeneration and repair of autologous soft and hard tissues through animal experiments.OBJECTIVE:To prepare advanced platelet-rich fibrin membrane and observe the histological changes of the diced cartilage grafts wrapped in advanced platelet-rich fibrin membrane and autogenous fascia.METHODS:The following same procedures were carried out in 12 New Zealand rabbits:preparation of advanced platelet-rich fibrin membrane,collection of cartilage tissue of the left ear and the fascia tissue of the right hind limb of the rabbit.There were three types of cartilage grafts:diced cartilage;diced cartilage wrapped with autogenous fascia;diced cartilage grafts wrapped with advanced platelet-rich fibrin membrane.The grafts then were placed into subcutaneous pockets prepared on the backs of the same rabbits.The grafts were removed,and subjected to general observation and histopathologic examination 4 months after surgery.RESULTS AND CONCLUSION:(1)The thickness of diced cartilage grafts wrapped with autogenous fascia and advanced platelet-rich fibrin membrane was thicker than diced cartilage grafts;the shape of the grafts was more rounded;and the surface was flatter.(2)The chondrocyte survival rate and regeneration potential of diced cartilage grafts wrapped with advanced platelet-rich fibrin membrane were significantly better than the other two grafts(P<0.05).There was no statistical difference in the other histological parameters among the three types of cartilage grafts.(3)The results show that advanced platelet-rich fibrin membrane can improve the survival rate of diced cartilage and graft shaping and reduce the resorption of diced cartilage after transplantation.

关 键 词：富血小板纤维蛋白 软骨 软骨移植 自体筋膜 移植物再吸收 蛋白质实验 材料 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学]