颗粒软骨加α-氰基丙烯酸正丁酯胶结合3D打印技术辅助制作个性化耳支架  

Granule cartilage plus n-butylα-cyanoacrylate glue combined with 3D printing technology for preparation of individualized ear scaffolds

作　　者：张帆 李高峰[2] 胡益高 蔡立宏 Zhang Fan;Li Gaofeng;Hu Yigao;Cai Lihong(Xiangtan Aist Medical Beauty Clinic,Xiangtan 411100,Hunan Provinee,China;Department of Plastic Laser Cosmetology,Hunan Provincial People's Hospital(the First Affiliated Hospital of Hunan Noirnal University),Changsha 410005,Hunan Province,China;Department of Radiology,The Third Hospital of Changsha,Changsha 410007,Hunan Province,China)

机构地区：[1]湘潭爱思特医疗美容门诊部,湖南省湘潭市411100 [2]湖南省人民医院(湖南师范大学附属第一医院)整形激光美容科,湖南省长沙市410005 [3]长沙市第三医院放射影像科,湖南省长沙市410007

出　　处：《中国组织工程研究》2020年第34期5520-5525,共6页Chinese Journal of Tissue Engineering Research

基　　金：湖南省教育厅科学研究项目一般项目(16C0964),课题名称:颗粒软骨加α-氰基丙烯酸正丁酯结合3D打印技术辅助制作耳支架的实验研究,项目负责人:李高峰。

摘　　要：背景:传统耳再造手术中耳支架的制作容易导致再造耳失真,3D打印技术使个性化、高仿真的小耳再造成为可能。目的:探讨颗粒软骨加α-氰基丙烯酸正丁酯结合3D打印技术制作个性化耳支架的可行性。方法:根据耳畸形患者健侧耳制作患侧耳的3D耳支架阳模和阴模,将颗粒软骨用α-氰基丙烯酸正丁酯粘连放入阴模中,同时对比阳模微调,制成耳软骨支架后,将其植入新西兰大白兔背部,动态观察再造耳郭的形态,术后4,8,12周分批取出耳支架,组织学观察颗粒软骨间连接情况、软骨的存活状况等,免疫组化观察Ⅱ型胶原表达。动物实验获得湖南师范大学实验动物福利伦理审查委员会批准(批准号:201517)。结果与结论:①大体观察:与术前比较,术后4,8,12周的再造耳郭长度、宽度、高度无明显变化,质量明显增加(P<0.05);②组织学观察:颗粒软骨周围可见炎性细胞浸润,随着标本植入时间延长,炎性细胞逐渐减少,附着支架的筋膜中有较多血管生成;随着植入时间的延长,软骨膜内新生软骨细胞逐渐增多,体积逐渐增大,成熟软骨细胞逐渐增多;软骨碎片之间的α-氰基丙烯酸正丁酯未见明显分解,绝大部分软骨片之间仍被α-氰基丙烯酸正丁酯间隔,少部分软骨片之间有纤维连接形成,随着植入时间延长,软骨片之间的连接有增多的趋势;③免疫组化学观察:随着标本植入时间延长,Ⅱ型胶原表达逐渐增强;④结果表明:颗粒软骨加α-氰基丙烯酸正丁酯结合3D打印模具可以制作个性化耳支架。BACKGROUND:The manufacture of ear brackets in traditional ear reconstruction is likely to cause ear distortion,and 3 D printing technology makes it possible to prepare individualized and highly simulated ears.OBJECTIVE:To explore the feasibility of making individualized ear scaffolds using granule cartilage plus n-butylα-cyanoacrylate glue with 3 D printing technology.METHODS:Male and female modes of 3 D ear scaffolds were prepared for the affected ears according to the ears on the non-injured side.Granule cartilage and n-butylα-cyanoacrylate glue were placed in the female modes to prepare ear cartilage scaffolds through slight adjustment based on male modes.Then the ear cartilage scaffolds were implanted into the back of New Zealand rabbits.The morphology of constructed ear scaffolds was dynamically observed.At postoperative 4,8,and 12 weeks,the ear scaffolds were taken out in batches.The connection between granule cartilage and cartilage survival was histologically observed.Immunohistochemistry was performed to detect the expression of type II collagen.The animal experiments were approved by the Animal Ethics Committee of Hunan Normal University,China(approval No.201517).RESULTS and CONCLUSION:(1)Gross observation:compared with before surgery,the length,width and height of the reconstructed ears were not significantly changed,but the weight of the reconstructed ears was significantly increased at 4,8,and 12 weeks after surgery(P<0.05).(2)Histological observation:Inflammatory cell infiltration was observed around granular cartilage.With the prolongation of ear scaffold implantation time,inflammatory cell count gradually decreased,and there were more newly formed vessels in the fascia attached to the scaffold.With the prolongation of implantation time,the number of new chondrocytes in the cartilage membrane gradually increased,cartilage volume gradually increased,and mature chondrocytes gradually increased.Obvious decomposition of n-butylα-cyanoacrylate between cartilage pieces was not observed.Most of the car

关 键 词：材料 颗粒软骨 Α-氰基丙烯酸正丁酯 耳支架 个性化 耳郭再造 3D打印 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]