改良的大鼠鼠尾增生性瘢痕动物模型制备方法  

An Improved Rat Tail Animal Model of Hypertrophic Scar

作　　者：徐祥文 邝依敏 黄昕[1] 高雅姗 李海洲[1] 顾舒晨 昝涛[1] XU Xiangwen;KUANG Yimin;HUANG Xin;GAO Yashan;LI Haizhou;GU Shuchen;ZAN Tao(Department of Plastic and Reconstructive Surgery,Shanghai Ninth People′s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,上海市200011

出　　处：《组织工程与重建外科杂志》2020年第1期22-26,38,共5页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金(81772086),上海市教育委员会高峰高原学科建设计划,上海市“医苑新星”杰出青年医学人才计划,上海交通大学“晨星-优秀青年学者奖励计划”(副教授支持计划A类)。

摘　　要：目的建立增生性瘢痕的有效动物模型。方法于SD大鼠鼠尾形成6 mm矩形创面,根据创面边缘距鼠尾根部的距离分为A组(3 cm)和B组(5 cm)。术后16 d进行牵拉并测量创面牵张力。于牵拉7、14、21、28 d拍照记录两组的瘢痕的大体变化。于牵拉28 d检测两组瘢痕中张力相关蛋白局部黏着斑激酶(FAK)和共激活因子相关蛋白(YAP)活化情况。结果A、B两组牵拉即刻的牵张力分别增加了(22.78%±2.452%)和(15.46%±2.193%)(P<0.05)。相比B组,A组瘢痕厚度显著增加(P<0.05),胶原束粗大呈漩涡样排列紊乱,且α-SMA蛋白表达高于B组(P<0.05)。A组FAK和YAP阳性表达率均较B组增高(P<0.05;P<0.001)。结论于鼠尾3 cm处建立创面进行牵拉可以建立更接近于人增生性瘢痕的动物模型。Objective To establish an effective animal model of hypertrophic scar.Methods A 6 mm square wound was made on the SD rat tail.According to the distance from the wound to the root of rat tail,the rats were randomly divided into group A(3 cm)and group B(5 cm).The mechanical stretch device was attached on the rat tail 16 days after operation and the strain was measured.Photos were taken at 7,14,21,28 days to observe the general appearance after stress induction.After 28 days of induction,histological staining were used to measure the expression of hypertrophic scar-associated protein.The activationof stress-associated protein,FAK and YAP were also investigated.Results The immediate tension of pulling in group A and group B increased(22.78%±2.452%)and(15.46%±2.193%),respectively(P<0.05).Compared with group B,scar thickness was significantly higher in group A(P<0.05).In group A,collagen bundles were thickened and distorted and the protein expression ofα-SMA was higher than in group B(P<0.05).The activation of FAK and the positive expression rate of YAP protein in nucleus in group A were higher than that in group B(P<0.05;P<0.001).Conclusion An animal model closer to human hypertrophic scar can be established by pulling the wound at 3 cm of the rat tail.

关 键 词：增生性瘢痕 张力 大鼠 局部黏着斑激酶 转录共激活因子相关蛋白 

分 类 号：R619.6[医药卫生—外科学]