柴胡三参胶囊对缺血性心律失常大鼠心肌细胞Bcl-2及Fas表达的影响  

Effect of Chaihu Sanshen Capsule on the Expression of Bcl-2 and Fas in Myocardial Cells of Rats with Ischemic Arrhythmia

作　　者：刘建和[1,2] 毛宗裕 肖冰凌 袁恒佑 杨成龙[1,2] 赵吉锐[1,2] Liu Jianhe;Mao Zongyu;Xiao Bingling;Yuan Hengyou;Yang Chenglong;Zhao Jirui(The First Affiliated Hospital of Hunan University of Chinese Medicine,Hunan,Changshu 410007,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学中医诊断学湖南省重点实验室,湖南长沙410007 [3]湖南中医药大学,湖南长沙410208

出　　处：《中国中医急症》2020年第6期962-965,977,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：湖南省中医药科研计划项目(201703);湖南省自然科学基金科卫联合项目(2018JJ6038);湖南省学位与研究生教育改革研究项目(2019JGYB163);湖南中医药大学中医学国内一流建设学科。

摘　　要：目的观察柴胡三参胶囊对缺血性心律失常大鼠心肌细胞Bcl-2及Fas蛋白的表达影响,进一步明确柴胡三参胶囊治疗缺血性心律失常的作用及机制。方法将实验大鼠随机分为假手术组、模型组、柴胡三参胶囊组、参松养心胶囊组、维拉帕米组、空白组,每组各10只。给予相应药物干预2周后观察各组的心律失常积分和Bcl-2、Fas表达的变化。结果柴胡三参胶囊组、参松养心胶囊组、维拉帕米组与模型组进行比较,心律失常评分下降(P <0.05),Bcl-2蛋白表达升高(P <0.01),Fas蛋白表达有一定程度降低(P <0.05)。结论柴胡三参胶囊能上调抑制凋亡因子Bcl-2表达,下调促进凋亡因子Fas表达,说明其可减轻细胞凋亡,缓解心肌损伤,为柴胡三参胶囊改善缺血性心律失常提供了实验依据。Objective:To observe the effect of Chaihu Sanshen Capsule on the expression of Bcl-2 and Fas protein in myocardial cells of rats with ischemic arrhythmia and to clarify the effect and mechanism of Bupleurum Sanshen capsule in the treatment of ischemic arrhythmia.Methods:The experimental rats were randomly divided into sham operation group,model group,Chaihu Sanshen Capsule group,Shensong Yangxin Capsule group,verapamil group,blank group,10 in each group.The changes of arrhythmia scores and Bcl-2,fas expression in the six groups were observed.Results:Compared with the model group,the arrhythmia scores of Chaihu Sanshen Capsule group,Shensong Yangxin Capsule group and verapamil group decreased significantly(P<0.05).The expression of Bcl-2 protein was increased,the drug intervention was effective,and the difference was statistically significant(P<0.01).Compared with the model group,the expression of Fas protein was reduced to a certain extent,and the difference was statistically significant(P<0.05).Conclusion:Chaihu Sanshen Capsule up-regulates and inhibits the expression of apoptosis factor Bcl-2,down-regulates and promotes the expression of apoptosis factor Fas,indicating that it can alleviate apoptosis and relieve myocardial injury,which provides experimental basis for Chaihu Sanshen Capsule to improve ischemic arrhythmia.

关 键 词：缺血性心律失常 心肌凋亡 柴胡三参胶囊 BCL-2 FAS 大鼠 

分 类 号：R285.5[医药卫生—中药学]