羧基化石墨烯/半胱胺修饰金电极对多巴胺的电化学行为研究  

Study on the Electrochemical Behavior of Dopamine at Carboxylated Graphene/Cysteamine Modified Gold Electrode

作　　者：高先娟 汲霞 王清路 杜青青 王凤丹 王怀生[2] GAO Xianjuan;JI Xia;WANG Qinglu;DU Qingqing;WANG Fengdan;WANG Huaisheng(Key Laboratory of Biomedical Engineering and Technology of Shandong High School,College of Pharmacy,Qilu Medical University,Shandong Zibo 255300,China;School of Chemistry and Chemical Engineering,Liaocheng University,Shandong Liaocheng 252000,China)

机构地区：[1]齐鲁医药学院药学院,山东省高校生物医学工程技术重点实验室,山东淄博255300 [2]聊城大学化学化工学院,山东聊城255000

出　　处：《生物技术进展》2020年第3期292-298,共7页Current Biotechnology

基　　金：山东省教育厅项目(J16LC51);淄博市基因编辑与细胞应用研究平台开放性项目(2019GECA016);齐鲁医药学院校级项目(X19ZKZC02)。

摘　　要：为了基于羧基化石墨烯/半胱胺修饰金电极建立更为先进的多巴胺生物传感器,以定量检测儿茶酚胺类神经递质多巴胺,利用自组装技术将半胱胺修饰于金电极上,再利用1-乙基-[3-二甲基氨基丙基]碳酰二亚胺盐酸化物/N-羟基琥珀酰亚胺(EDC/NHS)交联剂将羧基化石墨烯固定在修饰后的金电极上制成多巴胺电化学传感器。先对修饰电极进行表征以检验其灵敏度,再利用循环伏安法研究该电极在多巴胺溶液中的电化学行为,包括检测条件的优化和传感器性能的测定。修饰电极表征结果表明,羧基化石墨烯/半胱胺修饰金电极提高了电极传递电子的能力,具有较高的灵敏度。经单因素实验得出,最佳检测条件为利用pH 6.00的0.30 mol·L-1磷酸盐缓冲溶液(PBS)配制多巴胺溶液,扫描速率设定为200 mV·s-1。在最佳检测条件下,制备的多巴胺电化学传感器电流的大小随着多巴胺浓度的增大而增大,在1.0×10-3~3.5×10-3 mol·L-1范围内呈现良好的线性关系,线性回归方程为I=8.1206 C+7.017,相关系数R 2为0.9995。且该传感器精密度好,稳定性强,具有一定的抗干扰能力。研究结果为药物盐酸多巴胺注射液中多巴胺含量测定提供了支撑。In order to establish a more advanced dopamine biosensor based on the carboxylated graphene/cysteamine modified gold electrode for quantitative detection of catecholamine neurotransmitter dopamine,cysteamine was modified on gold electrode by self-assembly technology,and then the carboxylated graphene was fixed on the modified electrode by 1-ethyl-[3-dimethylaminopropyl]carbodiimide hydrochloride/N-hydroxysuccinimide(EDC/NHS)crosslinker to prepare the dopamine electrochemical sensor.Firstly,the modified electrode was characterized to test its sensitivity,and then the electrochemical behavior of the electrode in dopamine solution was studied by cyclic voltammetry,including optimization of detection conditions and determination of sensor performance.Characterization results of the modified electrode showed that the carboxylated graphene/cysteamine modified gold electrode improved the electron transfer ability of the electrode and had higher sensitivity.Through single factor experiments,the best detection condition was to use 0.30 mol·L-1 phosphate buffer solution(PBS)with pH 6.00 to prepare dopamine solution,and the scanning rate set as 200 mV·s-1.Under the optimal detection conditions,the magnitude of the current of the prepared dopamine electrochemical sensor increased with the increase of dopamine concentration,showing a good linear relationship in the range of 1.0×10-3~3.5×10-3 mol·L-1.The linear regression equation was I=8.1206 C+7.017,and the correlation coefficient R 2 was 0.9995.Moreover,the sensor had good precision,strong stability and certain anti-interference capability.The research results provided support for the determination of dopamine content in drug dopamine hydrochloride injection.

关 键 词：半胱胺 自组装 羧基化石墨烯 金电极修饰 多巴胺传感器 

分 类 号：O657.1[理学—分析化学] TQ460.72[理学—化学]