异齿裂腹鱼(Schizothorax oconnori)CLOCK蛋白结构和功能的生物信息学分析  

Bioinformatic Analysis of Structure and Function of Schizothorax oconnori CLOCK Protein

作　　者：周建设 张驰[1] 王万良 曾本和 王且鲁 王金林 ZHOU Jian-she;ZHANG Chi;WANG Wan-liang;ZENG Ben-he;WANG Qie-lu;WANG Jin-lin(Institute of Fisheries Science,Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet Lhasa 850032,China;College of Animal Science and Veterinary Medicine,Henan Agricultural University,Henan Zhengzhou 450000,China)

机构地区：[1]西藏自治区农牧科学院水产科学研究所,西藏拉萨850032 [2]河南农业大学牧医工程学院,河南郑州450000

出　　处：《西藏农业科技》2020年第1期11-16,共6页Tibet Journal of Agricultural Sciences

基　　金：西藏自治区自然科学基金项目“clock基因对异齿裂腹鱼繁殖行为微进化的响应研究”(XZ2019ZRG-103)。

摘　　要：为探讨异齿裂腹鱼CLOCK蛋白的结构、功能信息,本文通过生物信息学的方法对异齿裂腹鱼CLOCK蛋白的理化性质、亚细胞定位、跨膜区和信号肽、二级结构、三级结构、蛋白互作网络、系统进化等进行分析。异齿裂腹鱼CLOCK蛋白分子式为C4389H6978N1278O1405S37,相对分子量101315.62 Da,氨基酸含量最高的是谷氨酰胺为146个,半衰期为30 h,不稳定系数为68.37,属不稳定蛋白,理论等电点(PI)为6.44,属于酸性蛋白质,脂肪系数为69.96,CLOCK属于亲水性蛋白。蛋白均定位于细胞核内,不存在信号肽序列和跨膜区,存在一个核定位信号序列为"KAKRVSRNKSEKKRR"。异齿裂腹鱼CLOCK蛋白具有疏松的结构特征,含有111个磷酸化位点,CLOCK蛋白与多个时钟相关的蛋白互作,在物种进化上与金线鲃属鱼类CLOCK蛋白具有较高的同源性。To discuss the structure and functional information of Schizothorax oconnori CLOCK protein,in this paper,the physicochemical property,subcellular localization,transmembrane domain,signal peptides,secondary structure,tertiary structure,protein interaction network and phylogenetic of Schizothorax oconnori CLOCK protein were analyzed.The molecular formula of CLOCK protein was C 4389 H 6978 N 1278 O 1405 S 37 with 101315.62Da relative molecular weight.The Gln(Q)was the highest content with 146 number in all amino acid of CLOCK protein and had 30 hours half-life period,68.37 coefficient of instability,6.44 isoelectric point,69.96 coefficient of fat.The protein was acid,instability and hydrophilic and was located in nuclear,there was no signal peptide sequence and transmembrane domain,nuclear localization signal sequence was‘KAKRVSRNKSEKKRR’.Schizothorax oconnori CLOCK protein with architectural feature of loose contained 111 phosphorylation sites,and interacted with multi-the clock protein,in the evolution of species,the CLOCK protein of Sinocyclocheilus grahami has a high homology.

关 键 词：异齿裂腹鱼 CLOCK蛋白 生物信息学 

分 类 号：S965.199[农业科学—水产养殖]