Fe^3+诱导的DNA构象变化在基于AuNPs的DNA损伤比色检测中的应用  

Colorimetric Test of DNA Damage based on Gold Nanoparticle and Deformation of DNA Driven by Fe^3+

作　　者：林玲玲 许品生 Lin Lingling;Xu Pinsheng(Fujian Provincial Key Laboratory of Featured Materials in Biochemical Industry,Fujian Province University Key Laboratory of Green Energy and Environment Catalysis,College of Chemistry and Materials,Ningde Normal University,Ningde 352100;Academic Administration,Ningde Normal University,Ningde 352100,China)

机构地区：[1]宁德师范学院化学与材料学院,福建省特色生物化工材料重点实验室,绿色能源与环境催化福建省高等学校重点实验室,福建宁德352100 [2]宁德师范学院教务处,福建宁德352100

出　　处：《广东化工》2020年第11期22-24,共3页Guangdong Chemical Industry

基　　金：福建省自然科学基金项目(2019J01838);福建省中青年教师教育科研项目(JT180605);宁德师范学院科研资助项目(2018Y03,2018Q106)。

摘　　要：通过研究Fe^3+对热变性小牛胸腺DNA(ssDNA)构象变化的影响,建立基于Au纳米粒子(AuNPs)的DNA损伤的比色检测新方法。当ssDNA被氧化时,Fe^3+对ssDNA构象变化的影响将会减弱,从而影响AuNPs的聚集及显色。因此通过对AuNPs颜色变化的"肉眼"观察或吸收光谱测试可实现对ss DNA损伤的检测。Based on Au nanoparticles(AuNPs)and the conformation change of thermally denatured calf thymus DNA(ssDNA)driven by Fe^3+,a new method of colorimetric test for DNA oxidation damage was established.As the ssDNA was oxidized,the effect of the conformation change of ssDNA driven by Fe^3+would be weakened,and thus altered the aggregation and the coloration of AuNPs.Therefore,the ssDNA damage would be able to be detected by the“naked-eye”observation of color change or absorption spectrum of AuNPs.

关 键 词：构象变化 DNA 金纳米粒子 比色法 FE^3+ 

分 类 号：TQ[化学工程]