叉叶茅膏菜组培快繁的研究  

Study on Rapid Propagation of Sundew Tissue Culture

作　　者：许凤[1,2,3] 张艺萍 宋杰[1,2,3] 杨秀梅 王丽花[1,2,3] 苏艳 张丽芳[1,2,3] 瞿素萍 XU Feng;ZHANG Yi-ping;SONG Jie;YANG Xiu-mei;WANG Li-hua;SU Yan;ZHANG Li-fang;QU Su-ping(Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;National Engineering Research Center for Ornamental Horticulture,Kunming 650205,China;Yunnan Flower Breeding Key Laboratory,Kunming 650205,China)

机构地区：[1]云南省农业科学院花卉研究所,云南昆明650205 [2]国家观赏园艺工程研究中心,云南昆明650205 [3]云南省花卉育种重点实验室,云南昆明650205

出　　处：《江西农业学报》2020年第6期47-51,共5页Acta Agriculturae Jiangxi

基　　金：云南省国际科技合作项目“两种新特花卉的引进与繁育关键技术研发”(2018IA049)。

摘　　要：以叉叶茅膏菜花序为试验材料,利用器官脱分化再分化发生途径,对影响叉叶茅膏菜快繁体系的关键环节进行了研究,结果表明:叉叶茅膏菜外植体最佳消毒处理方式:先用洗衣粉水清洗1遍,在自来水下冲洗15~20 min,75%酒精处理30 s后用0.15%氯化汞溶液消毒8 min,最后用0.5%次氯酸混合液处理15 min;诱导愈伤组织的最佳培养基:MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+蔗糖30 g/L、MS+KT 1.0 mg/L+NAA 0.1 mg/L;最适宜的愈伤组织分化培养基:MS+ZT 0.5 mg/L+NAA 0.1 mg/L,分化出的芽健壮,芽数较多。In this study,the sundew inflorescences were used as the test material.By means of organ dedifferentiation and re-differentiation,the key link of the rapid propagation system of sundew was studied.The results showed that the best disinfection treatment method of the explants was as follows:washing with powder water for a time firstly,washing in tap water for 15~20 minutes,sterilizing for 30 s with 0.15%of mercuric chloride solution for 8 minutes,and then treating with 0.5%hypochlorous acid mixed solution for 15 min.The best medium for inducing callus was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L and MS KT 1.0 mg/L+NAA 0.1 mg/L.The most suitable medium for calli differentiation was MS+ZT 0.5 mg/L+NAA 0.1 mg/L.

关 键 词：叉叶茅膏菜 组培 快繁 

分 类 号：Q949.749.4[生物学—植物学] Q813.11