消瘀泄浊饮对慢性环孢素肾病大鼠ACE2-Ang(1-7)-MAS轴相关组分表达的影响  被引量:5

Effect of Xiaoyu Xiezhuo Drink on the Expression of ACE2-Ang(1-7)-MAS Axis Components in Rats with Chronic Cyclosporine Nephropathy

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作  者:鲁科达[1] 张冰冰[2] 石承乾 张培培[1] 叶黎青[1] LU Keda;ZHANG Bingbing;SHI Chengqian(The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,China;Zhejiang Chinese Medical University)

机构地区:[1]浙江中医药大学附属第一医院,杭州310006 [2]浙江中医药大学

出  处:《浙江中医药大学学报》2020年第5期407-414,共8页Journal of Zhejiang Chinese Medical University

基  金:浙江省自然科学基金青年项目(LQ16H270002);国家中医临床研究基地业务建设科研专项(JDZX2015117)。

摘  要:[目的]观察消瘀泄浊饮对慢性环孢素肾病大鼠血管紧张素转化酶2-血管紧张素(1-7)[angiotensin converting enzyme 2-angiotensin(1-7),ACE2-Ang(1-7)]-MAS轴相关组分表达的影响。[方法]40只雄性SD大鼠按随机数字表法分为空白对照组、模型组、消瘀泄浊饮组和贝那普利组,每组各10只。采用3mg·mL-1的环孢素A溶液灌胃及低盐饮食建模,灌胃4周后检测大鼠血肌酐(serum creatinine,Scr)、血尿素氮(blood urea nitrogen,BUN)水平,并计算内生肌酐清除率(creatinine clearance rate,Ccr);以苏木精-伊红(hematoxylin-eosin,HE)染色、Masson染色观察大鼠肾组织病理学改变,免疫组化法检测肾组织ACE2、Ang(1-7)和Ⅳ型胶原(collagenⅣ,ColⅣ)的表达;以酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)法检测血清及肾组织ACE2、Ang(1-7)和肾素含量。[结果]与空白对照组比较,模型组大鼠BUN、Scr水平明显升高,Ccr明显下降(均P<0.01);与模型组比较,消瘀泄浊饮组和贝那普利组大鼠BUN、Scr水平和Ccr明显改善(P<0.01,P<0.05,P<0.05)。与空白对照组比较,模型组肾组织ColⅣ阳性染色面积、总光密度明显升高(均P<0.01),而ACE2、Ang(1-7)阳性染色面积、总光密度均升高(P<0.01,P<0.05);与模型组比较,消瘀泄浊饮组和贝那普利组肾组织ColⅣ阳性染色面积、总光密度下降(均P<0.01),肾组织ACE2、Ang(1-7)阳性染色面积、总光密度明显升高(均P<0.05)。ELISA检测显示,与空白对照组比较,模型组肾组织和血清中肾素含量均显著升高(P<0.01,P<0.01),肾组织中ACE2含量显著升高(P<0.05),Ang(1-7)含量显著下降(P<0.05);血清ACE2、Ang(1-7)含量显著升高(均P<0.01)。与模型组比较,贝那普利组肾组织中肾素含量降低(P<0.05),而消瘀泄浊饮组和贝那普利组血清中肾素含量也降低(均P<0.05);两组肾组织ACE2含量升高(P<0.05),血清ACE2含量下降(P<0.01);而肾组织和血清Ang(1-7)含量均升高(均P<0.05);肾组织炎性细胞浸润[Objective]To observe the effect of Xiaoyu Xiezhuo Drink on angiotensin converting enzyme 2-angiotensin(1-7)[ACE2-Ang(1-7)]-MAS axis components in chronic cyclosporine nephropathy(CCN)rats.[Methods]Forty male SD rats were divided into 4 groups:Blank control group,model group,Xiaoyu Xiezhuo Drink group,Benazepril group.The rats model were induced by intragastric administration of 3 mg·mL-1 cyclosporine A(CsA)solution accompanied with low salt diet.After 4 weeks,the levels of blood urea nitrogen(BUN),serum creatinine(Scr)were detected by automatic biochemistry analyzer,and creatinine clearance rate(Ccr)was calculated.Pathological changes of renal tissue were observed by hematoxyli-eosine(HE)and Masson staining.The expression of ACE2,Ang(1-7)and collagenⅣ(ColⅣ)in renal tissue were detected by immunohistochemistry.The ACE2,Ang(1-7)and renin contents in serum and renal tissue was detected by enzyme-linked immunosorbent assay(ELISA).%[Results]Compared with blank control group,the levels of BUN,Scr in model group increased significantly,while Ccr decreased significantly(all P<0.01),compared with model group,the levels of BUN,Scr and Ccr in Xiaoyu Xiezhuo Drink group and Benazepril group were improved significantly(P<0.01,P<0.05,P<0.05).Compared with blank control group,the expression of ColⅣin renal tissue in model group were significantly up-regulated(P<0.01),while the expression of ACE2,Ang(1-7)in renal tissue were significantly up-regulated(P<0.01,P<0.05);compared with model group,the expression of ColⅣin renal tissue in Xiaoyu Xiezhuo Drink group and Benazepril group were significantly down-regulated(all P<0.01)and the expression of ACE2,Ang(1-7)in renal tissue were significantly up-regulated(all P<0.05).ELISA showed that compared with blank control group,the content of renin in serum and renal tissue of model group increased(P<0.01,P<0.01),while the content of ACE2 increased in renal tissue(P<0.05),Ang(1-7)decreased(P<0.05);the content of ACE2 and Ang(1-7)in serum increased(all P<0.01).Compared with model

关 键 词:消瘀泄浊饮 慢性环孢素肾病 ACE2 Ang(1-7) MAS Ⅳ型胶原 肾间质纤维化 

分 类 号:R331[医药卫生—人体生理学]

 

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