固态纳米孔上端粒序列的共价修饰与G-四联体折叠监测  

Covalent Modification of Telomere Sequence on Solid-State Nanopore and Monitoring for the G-Quadruplexes Folding Process

作　　者：王森[1,2] 梁丽媛 唐婧[1,2] 蔡瑶 翁婷 尹雅洁 王亮 王德强[1,2] Wang Sen;Liang Liyuan;Tang Jing;Cai Yao;Weng Ting;Yin Yajie;Wang Liang;Wang Deqiang(Chongqing Institute of Green and Intelligent Technology,Chinese Academy of Sciences,Chongqing 400714,China;University of Chinese Academy of Sciences,Beijing 100049,China;College of Instrumentation&Electrical Engineering,Jilin University,Changchun 130012,China)

机构地区：[1]中国科学院重庆绿色智能技术研究院,重庆400714 [2]中国科学院大学,北京100049 [3]吉林大学仪器科学与电气工程学院,长春130012

出　　处：《微纳电子技术》2020年第6期483-491,共9页Micronanoelectronic Technology

基　　金：中国科学院青年创新促进会项目(2017392);重庆市基础科学与前沿技术研究重点项目(cstc2017jcyjB0105)。

摘　　要：由于某些阳离子的存在,端粒序列可以自发地形成鸟嘌呤-四联体(G-四联体)。而G-四联体与人体的一些生理过程相关,因此可以作为一些肿瘤疾病的治疗靶标。提出了一种在限域空间内,利用固态纳米孔单分子动态监测人体端粒序列脱氧核糖核酸(DNA)在金属离子诱导下形成G-四联体的方法。通过将端粒序列DNA共价修饰在纳米孔内表面,在不同金属离子电导液体系中诱导端粒序列DNA折叠成不同物理尺寸的G-四联体,并能检测到外电场力作用下G-四联体构型的稳定时间,从而实现单分子水平动态监测G-四联体的折叠与解折叠过程。Telomere sequences can spontaneously form guanine-quadruplexes(G-quadruplexes)due to the presence of certain cations.G-quadruplexes are related to some physiological processes in the human body,and can be used as therapeutic targets for some tumor related diseases.By using the solid-state nanopore single molecule,an approach was presented to dynamically monitor the formation of G-quadruplexes with the human telomere sequence DNA induced by metal ions in the confined space.Through covalent modification of telomere sequence DNA on the inner surface of the nanopore,the telomere sequences DNA were induced to fold into G-quadruplexes with different physical sizes in different metal ionic conductivity fluids.The stability time of the G-quadruplexes configuration under external electric field force was detected,and the dynamic monitoring of Gquadruplexes folding and unfolding processes at single-molecule level was realized.

关 键 词：G-四联体 端粒序列DNA 固态纳米孔 共价修饰 单分子检测 

分 类 号：TN307[电子电信—物理电子学]