甘草素对来曲唑诱导的多囊卵巢综合征大鼠的保护作用及机制研究  

The protection and mechanism ofLicorice on symptoms of polycytic ovary syndrome in Letrozole-induced rats

作　　者：江璐[1] 蔡冬明[1] 张海洲[1] JIANG Lu;CAI Dong-ming;ZHANG Hai-zhou(Faculty of pharmaceutical sciences,The First Affiliated Hospital to Soochow University,Jiangsu Suchoow215006,China)

机构地区：[1]苏州大学附属第一医院药学部,江苏苏州215006

出　　处：《中国医院药学杂志》2020年第7期791-794,799,共5页Chinese Journal of Hospital Pharmacy

基　　金：江苏省自然科学基金项目(编号:BK20170386)。

摘　　要：目的:探究甘草素对来曲唑诱导的多囊卵巢综合征(PCOS)大鼠的保护作用及机制研究。方法:将40只雌性SD大鼠随机分为4组:A组:不做任何处理,B组:造大鼠多囊卵巢综合征模型,C组:给予甘草素300 mg·kg^-1,D组:给予甘草素100 mg·kg^-1。末次给药结束12 h后,称取大鼠卵巢质量;采用HE染色的方法观察大鼠卵巢组织形态的变化;采用ELISA法检测血清中促卵泡雌激素(FSH)、促黄体生成素(LH)、雌二醇(E2)、孕酮(P)和睾酮(T)含量变化;采用Western blot法检测各组大鼠卵巢组织中P450芳香化酶(P450arom)的表达量。结果:大鼠卵巢质量测定结果表明,C组较B组大鼠卵巢质量降低(P<0.05)。HE染色结果表明,B组大鼠的卵巢组织形态学出现异常改变,C组较B组卵巢组织形态有明显改善,D组大鼠卵巢组织形态未见明显改善。ELISA结果表明,B组与A组大鼠血清中LH、T、E2含量比较,差异有显著性(P<0.05);C组与B组大鼠血清中LH、T、E2含量比较,差异有显著性(P<0.05)。PCR结果表明,C组大鼠卵巢组织中CYP19 mRNA含量较B组降低(P<0.05)。WB结果表明,B组大鼠卵巢组织中P450arom蛋白表达水平比A组增多(P<0.05);C组大鼠卵巢组织中P450arom蛋白表达水平比B组减少(P<0.05);D组与B组大鼠卵巢组织中P450arom蛋白相比无明显变化(P>0.05);C组大鼠卵巢组织中P450arom蛋白表达水平比D组降低(P<0.05)。结论:甘草素可明显改善来曲唑诱导的多卵巢综合征大鼠的卵巢组织形态,调节性激素分泌,抑制卵巢组织中P450arom的表达。OBJECTIVE To explore the protective effect and mechanism of Licorice on symptoms of polycystic ovary syndrome in Letrozole-induced rats.METHODS 40 female SD rats were selected and randomly divided into four groups:Normal group(group A),Model(group B),300 mg·kg^-1 Licorice(group C)and 100 mg·kg^-1 Licorice(group D).At the 12 hours after the last administration,the rat ovary was weighed and the pathological changes of the ovary were observed through HE staining.Then,changes in serum follicular estrogen(FSH),luteinizing hormone(LH),estradiol(E2),progesterone(P)and testosterone(T)were detected by ELISA.Finally,Western Blot was used to detect the expression of P450arom.RESULTS The weight of left and right ovary tissue in group C was significantly lower than that in group B(P<0.05).The results of HE staining showed that the morphology of ovarian tissue in group B was abnormally changed.The morphology of ovarian tissue in group C was significantly improved compared with group B.The morphology of ovarian tissue in group D was not improved.The data of ELISA shows that the content of LH,T,E2 have statistically significant difference between group B and group A(P<0.05),and the content of LH,T,E2 have statistically significant difference between group C and group B(P<0.05).The result of PCR shows that the expression of CYP19 mRNA in group C was statistically significantly lower than group B(P<0.05).Western blot results showed that the expression levels of P450arom in group B was higher than group A(P<0.05),and the expression levels of P450arom in group C was lower than group B(P<0.05).While there was no statistically significant difference in the expression levels of P450arom between group D and group B(P>0.05).And the expression levels of P450arom in group C was lower than group D(P<0.05).CONCLUSION Glycyrrhizin can obviously improve the morphology of ovarian tissue,regulate the secretion of sex hormone and inhibit the expression of P450arom in ovarian tissue of rats with polyovarian syndrome induced by letrozole.

关 键 词：甘草素 来曲唑 多囊卵巢综合征 P450芳香化酶 性激素 

分 类 号：R285[医药卫生—中药学]