软珊瑚共附生真菌Aspergillus sp.EGF15-0-3的柳珊瑚酸类代谢产物研究  

Researches on the Subergane-Type Sesquiterpenes from the Soft Coral-Derived Fungus Aspergillus sp.EGF15-0-3

作　　者：冯婵 韦霞 胡金姗 王思玉 刘炳新[1] 谢珍玉[2] 荣莉[1] 李小翚[1] 张翠仙[1] Feng Chan;Wei Xia;Hu Jinshan;Wang Siyu;Liu Bingxin;Xie Zhenyu;Rong Li;Li Xiaohui;Zhang Cuixian(School of Pharmaceutical Science,Guangzhou University of Chinese Medicine,Guangzhou 510006;College of Marine Science,Hainan University,Haikou 570228)

机构地区：[1]广州中医药大学中药学院,广州510006 [2]海南大学药学院,海口570228

出　　处：《有机化学》2020年第5期1275-1280,共6页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.81741160);广东省自然科学基金(No.2014A030313411);广东省科技计划(Nos.2017A020217008,2015A020216017);广东省自然资源厅广东省海洋经济发展海洋六大产业专项资金(No.粤自然资合[2020]039);广东省中药药效物质基础和创新药物研究重点实验室开放课题、广州中医药大学薪火计划重点项目(No.XH20170110);海南大学南海海洋资源利用国家重点实验室开放课题(No.2016003)资助项目.

摘　　要：首次从软珊瑚共附生曲霉属真菌Aspergillus sp.EGF15-0-3中分离得到可逆性胆碱酯酶抑制剂环戊烷倍半萜类化合物柳珊瑚酸(1)及其类似物2-脱氧-2β-羟基柳珊瑚酸(2),并确定其结构.根据单菌株多次级代谢产物(OSMAC)策略和富营养化培养,在23种不同的培养基中首次应用全球天然产物分子网络(GNPS)检测1和2是否存在,并选出1和2的最佳培养基组成.结果表明,除马铃薯葡萄糖肉汤(PDB)培养基外,1还存在其他6种培养基中,2的含量在液体和大米培养基中不同,其中GPY+CaCO3培养基为1和2的最佳培养基.首次证实软珊瑚共附生曲霉属真菌EGF15-0-3是1和2的可能微生物来源,同时也验证了海洋无脊椎动物产生新颖的具有生物活性的次级代谢产物通常来自内生、附生、共生或环境微生物的假说.Subergorgic acid(1),a cyclopentapentalane sesquiterpenoid and potential reversible cholinesterase inhibitor,and its analogue 2-deoxy-2β-hydroxysubergorgic acid(2)were first isolated from soft coral-associated symbiotic and epiphytic fungus Aspergillus sp.EGF15-0-3.Their structures were established by NMR and HRESIMS spectroscopic data.According to the strategy of one strain many compounds(OSMAC)and eutrophic culture,Global Natural Products Molecular Networking(GNPS)was applied to detect the presence of compounds 1 and 2 in 23 different media for optimal medium compositions.As a result,compound 1 was found in 6 different media besides potato dextrose broth(PDB)medium,the contents of 2 were different in the solid and liquid media,and the best medium for compounds 1 and 2 was GPY+CaCO3.It was demonstrated that the soft coral-associated symbiotic and epiphytic fungus Aspergillus sp.EGF15-0-3 was the microbiological producer of compounds 1 and 2.This work provided strong evidence to support the hypothesis that novel and bioactive secondary metabolites produced by marine invertebrates often come from endogenetic,epiphytic,symbiotic or environmental microorganisms.

关 键 词：软珊瑚附生真菌 曲霉属真菌Aspergillus sp.EGF15-0-3 柳珊瑚酸 GNPS 

分 类 号：TQ464[化学工程—制药化工]