蝙蝠蛾拟青霉复合胶囊对四氯化碳与酒精所致小鼠急性肝损伤保护作用  

Protective effect of Paecilomyces hepialid compound capsule on acute liver injury induced by carbon tetrachloride and alcohol in mice

作　　者：陈頔 唐艳斌 朴玮 王鸥 黄建 王丽娟 CHEN Di;TANG Yan-bin;PIAO Wei(National Institute for Nutrition and Health,Chinese Center for Disease Control and Prevention,Beijing 100050,China)

机构地区：[1]中国疾病预防控制中心营养与健康所,北京100050

出　　处：《中国公共卫生》2020年第5期739-744,共6页Chinese Journal of Public Health

摘　　要：目的探讨蝙蝠蛾拟青霉复合胶囊对四氯化碳与酒精所致小鼠急性肝损伤的保护作用。方法分别采用四氯化碳和酒精灌胃诱导小鼠急性肝损伤模型,测定四氯化碳急性肝损伤模型小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的含量及酒精急性肝损伤模型小鼠肝组织中丙二醛(MDA)、甘油三酯(TG)、还原型谷胱甘肽(GSH)的含量并观察2组肝组织切片病理形态学变化。结果蝙蝠蛾拟青霉复合胶囊675、2025 mg/kg BW剂量组四氯化碳急性肝损伤小鼠血清的ALT、AST含量均低于模型对照组,差异具有统计学意义(P<0.05)。急性酒精性肝损伤模型中,675、2025 mg/kg BW剂量组的MDA含量和3个剂量组的TG含量明显低于模型对照组,675、2025 mg/kg BW剂量组的GSH含量明显高于模型对照组,差异均具有显著性(P<0.05)。小鼠肝组织形态学结果表明,四氯化碳肝损伤模型肝组织中、高剂量组的肝脏细胞坏死程度与模型对照组比较积分显著下降,差异具有显著性(P<0.05),各剂量组气球样变积分和高剂量组水样变程度积分也较模型组有显著性降低(P<0.01);蝙蝠蛾拟青霉复合胶囊能显著改善急性酒精性肝损伤小鼠肝细胞脂肪变性程度,差异具有显著性(P<0.05)。结论蝙蝠蛾拟青霉复合胶囊对小鼠急性肝损伤具有较好的辅助保护功能。Objective To study protective effect of Paecilomyces hepialid compound capsule(PHCC,mainly composed of Paecilomyces hepialid,Lucid ganoderma,Pueraria lobata,and taurine)on acute liver injury(ALI)induced by carbon tetrachloride(CCL4)and alcohol in mice.Methods Totally 100 specific pathogen free Kunming mice were divided into two subgroups for establishment of intragastric administration CCL4(0.6%,5 mL/kg body weight[BW])or alcohol(50%,13 mL/kg BW)induced ALI model.The 50 mice in each subgroup were assigned into 5 groups(10 for each group):a blank control and a model control with intragastric administration of distilled water,and low-,moderate-,and high-PHCC groups with intragastric administration of PHCC at doses of 337.5,675,and 2025 mg/kg BW once a day continuously for 36 days,respectively.Serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)of the mice with CCL4-induced ALI were determined.Contents of malondialdehyde(MDA),triglyceride(TG),and reduced glutathione(GSH)in liver tissue of mice with alcohol-induced ALI were measured.Histopathological changes in liver tissue were observed in all the rats.Results The serum ALT and AST of the CCL4-induced ALI mice with moderate-and high-PHCC were significantly lower than those of the model control mice(all P<0.05).Among the alcohol-induced ALI mice,significantly lower MDA in the mice with moderate-and high-PHCC treatment and TG and in all PHCC treated mice but higher GSH in the mice with moderate-and high-PHCC treatment were detected in comparison with those in the model control mice(P<0.05 for all).Liver tissue morphology observations of CCL4-induced ALI mice revealed significantly decreased scale for cell necrosis in the mice with moderate-and high-PHCC treatment(P<0.05),reduced scale for ballooning change in all PHCC treated mice,and declined scale for watery degeneration in high-PHCC treated mice(both P<0.01)compared to those in the model control mice.Liver cell steatosis was significantly alleviated in the alcohol-induced ALI mice with PHCC treatm

关 键 词：蝙蝠蛾拟青霉复合胶囊胶囊 酒精 四氯化碳 急性肝损伤 保肝功能 

分 类 号：R99[医药卫生—毒理学]