大豆响应耐涝的MAPK信号通路相关基因及蛋白的鉴定分析  

Identification of Genes and Proteins Involved in MAPK Pathway Response to Submergence Stress in Soybean

作　　者：林延慧 唐力琼[1] 徐靖[1] 徐冉[2] 韩义胜[1] 王新华 林昊 朱红林[1] 王效宁[1] Lin Yanhui;Tang Liqiong;Xu Jing;Xu Ran;Han Yisheng;Wang Xinhua;Lin Hao;Zhu Honglin;Wang Xiaoning(Hainan Key Laboratory of Crop Genetics and Breeding,Hainan Scientific Research Station of Crop Gene Resource&Germplasm Enhancement,Ministry of Agriculture,Institute of Food Crops,Hainan Academy of Agricultural Sciences,Haikou,571100;Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan,250100)

机构地区：[1]海南省农业科学院粮食作物研究所,海南省农作物遗传育种重点实验室,农业部作物基因资源与种质创制海南科学观测实验站,海口571100 [2]山东省农业科学院作物研究所,济南250100

出　　处：《分子植物育种》2020年第12期3819-3824,共6页Molecular Plant Breeding

基　　金：2019年海南省基础与应用基础研究计划(自然科学领域)高层次人才项目基金资助(2019RC357);海南省农业科学院省属科研院所技术开发研究专项(琼科[2019]69号)共同资助。

摘　　要：大豆是一种耐涝性较差的作物,涝害会引起大豆产量下降,甚至绝产,而大豆耐涝的遗传机制尚不明确。本研究通过转录组和蛋白组的关联分析,筛选出3个大豆响应耐涝的MAPK信号通路相关的差异表达基因/蛋白,转录组数据分析结果显示,Glyma.05G124000在4个时间点的表达量变化最为明显,对其进行RT-qPCR分析,验证了转录组数据的可靠性。进而对Glyma.05G124000的理化性质和保守结构域进行分析,发现组成该蛋白的氨基酸中亮氨酸(Leu)所占的比例最高,该蛋白内部含有多个LRR保守结构域,蛋白的三级结构展示了LRR保守结构域中所有Leu的位置。本研究筛选到的3个基因都参与编码FLS2蛋白,信号肽预测结果发现Glyma.05G124000含有信号肽,根据MAPK信号通路图,我们推测涝害胁迫引发了植物类似于病原菌侵染的基本防御反应,传递信号通过FLS2蛋白进一步激活了MAPK信号通路来抵御涝害胁迫。本研究为探索大豆的耐涝机制,提高涝害条件下大豆的产量提供了理论基础和基因资源。Soybean is a kind of crop with poor submergence resistance.Submergence can cause decrease in soybean yield,but the genetic mechanism of submergence resistance is not clear.In this study,three genes/proteins related to MAPK signaling pathway responding to submergence resistance were isolated through association analysis of transcriptome and proteome.The result of transcriptome data analysis showed that Glyma.05g124000 had the most obvious change in expression level at four time points,and RT-qPCR analysis was performed to verify the reliability of transcriptome data.Further,the physicochemical property and conserved domains of Glyma.05g124000 protein were analyzed,and it was found that leucine(Leu)accounted for the highest proportion in this protein,which contained multiple LRR conserved domains.The tertiary structure showed the locations of all Leus in LRR conserved domains. All three genes selected in this study encoded FLS2 protein. The prediction ofsignal peptide found that Glyma.05g124000 contained signal peptide. According to the MAPK signaling map, wespeculated that submergence stress triggered the basic defense response, which was similar to pathogen infection inplants, and the signaling further through FLS2 protein activated the MAPK signaling pathway to resist submergencestress. This study provided the theoretical basis and gene resources for exploring the mechanism of submergenceresistance and improving the yield of soybean.

关 键 词：大豆(Glycine max) 耐涝 转录组 蛋白组 MAPK信号通路 

分 类 号：S565.1[农业科学—作物学] S422