‘Y两优2号’叶片叶绿素含量QTL的定位  

QTL Mapping of Leaf Chlorophyll Content in 'Y-Liang-You2'

作　　者：李盼 常硕其[2] 常天根 欧阳翔 吴俊[2] 邓启云[2,4] 夏石头 朱新广[3] Li Pan;Chang Shuoqi;Chang Tiangen;Ouyang Xiang;Wu Jun;Deng Qiyun;Xia Shitou;Zhu Xinguang(Hunan Provincial Key Laboratory of Phytohormones&Growth Development,Changsha,Hunan Agricultural University,410128;Hunan Hybrid Rice Research Center,Changsha,410125;Shanghai Institute of Physiology and Ecology,Chinese Academy of Sciences,Shanghai,200031;Bio-Rice(Hunan)Co.Ltd.,Changsha,410100)

机构地区：[1]湖南农业大学,植物激素与生长发育湖南省重点实验室,长沙410128 [2]湖南杂交水稻研究中心,长沙410125 [3]中国科学院上海生理生态研究所,上海200031 [4]湖南袁创超级稻技术有限公司,长沙410100

出　　处：《分子植物育种》2020年第12期3944-3951,共8页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2017YFD0301502);湖南省自然科学基金项目(2018JJ2286);湖南省农业科学院创新项目(2017JC04)共同资助。

摘　　要：优化叶片叶绿素含量有利于提高冠层光合效率。第三期超级稻品种‘Y两优2号’(YLY2)是由父本‘远恢2号’(YH2)与母本‘Y58S’杂交而成的两系法杂交水稻。‘YLY2’与其父本‘YH2’叶片均具有低叶绿素含量的特性。为鉴定控制‘YLY2’叶绿素含量的关键基因,本研究以‘YLY2’构建的高世代重组自交系(RIL)为材料,利用基因组重测序方法构建了该群体的高密度遗传图谱;通过多年测量不同生长发育阶段的叶片叶绿素含量(SPAD)值,开展数量性状位点(quantitative trait locus,QTL)定位。本研究鉴定出了控制叶片SPAD的15个潜在QTL,并确定了其遗传效应值。研究发现的15个QTL中有4个与以往的研究位于相似位点,同时也发现了全新QTL位点,为鉴定控制‘YLY2’叶绿素含量的关键基因提供了一定的科学依据。Optimizing leaf chlorophyll content can improve canopy photosynthetic efficiency.The third phase super-rice cultivar'Y-Liang-You2'(YLY2)is a two-line hybrid rice from crossing of its male parent,'Yuan-Hui2'(YH2)and its female parent'Y58S'.Both'YLY2'and its male parent'YH2'have low leaf chlorophyll content.In order to mine key genes controlling chlorophyll content of'YLY2',we constructed a high generation recombinant inbred lines(RIL)population of'YLY2'and obtained high-density genetic map of this population by genome re-sequencing.QTL mapping of leaf chlorophyll content was performed based on leaf SPAD value which was measured at different growth and development stages for many years.As a result,15 QTLs that potentially control leaf SPAD value were identified;Their genetic effect value were determined as well.4 of the 15 QTLs identified in this study are located at similar sites as previous reports,and the others were reported for the first time,all of which laid a foundation for mining key genes controlling the chlorophyll content of'YLY2'.

关 键 词：超级稻 ‘Y两优2号’ 叶绿素含量 QTL 冠层光合 

分 类 号：S511[农业科学—作物学]