痰湿质肥胖人群腹型肥胖指标与外周血T辅助细胞极化变化的关联  

Relationship Between the Change of T Helper Cell and Abdominal Obesity Related Indicators in Obesity Patients with Phlegm-Dampness Constitution

作　　者：曾慧妍 杨彩凤 周钦云 张锦明 朱娅莉 陈裕 ZENG Hui-yan;YANG Cai-feng;ZHOU Qin-yun;ZHANG Jin-ming;ZHU Ya-li;CHEN Yu(The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou Guangdong 510120,China)

机构地区：[1]广州中医药大学第二附属医院,广东广州510006

出　　处：《中医药导报》2020年第9期82-84,92,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广东省科技厅-广东省中医药科学院联合科研专项(2017A020213026)。

摘　　要：目的:观察痰湿质肥胖人群腹型肥胖相关指标与其外周血T辅助细胞(T helper cells, Th)亚群比例变化的相关性。方法:共入选61例病例,根据中医体质评判标准及体重指数(body mass index,BMI)分为痰湿质肥胖组32例及非痰湿质肥胖组29例,另纳入30名BMI正常且中医体质辨识为平和质的健康人作为平和质正常对照组。采用流式细胞仪单克隆免疫荧光法分选Th1、Th2细胞并计算其比值,生物电阻抗检测内脏脂肪面积,皮脂厚度计测量腹部皮下脂肪厚度,测量身高、腰围(WC)并计算腰臀比(WHR)、腰高比(WHtR)等腹型肥胖指标,进一步分析这些腹型肥胖指标与Th1/Th2比值的关联性。结果:痰湿质肥胖组的Th1细胞比率、Th1/Th2比值均显著高于非痰湿质肥胖组、平和质正常对照组(P<0.05);痰湿质肥胖组、非痰湿质肥胖组的Th2比率均明显低于平和质正常对照组(P<0.05)。相关分析结果显示,痰湿质肥胖组的内脏脂肪面积、腹部皮下脂肪厚度、WC、WHR及WHtR分别与Th1/Th2比值呈正相关关系(r=0.509,r=0.714,r=0.788,r=0.491,r=0.778,P<0.05)。结论:痰湿质肥胖患者体内均存在明显的Th1/Th2细胞比率失衡且与腹部脂肪堆积及腹型肥胖程度明显相关。这可能是痰湿质人群具有更高的腹型肥胖患病率的原因之一。Objective:To explore the relationship between the changes of drift and proportion of Th1/Th2 cell subsets and abdominal obesity related indicators in obesity patients with phlegm-dampness constitution.Methods:According to Traditional Chinese Medicine(TCM)constitution and standard and BMI,61 obese cases were divided into obesity phlegm-dampness constitution group(32 cases)and obesity non phlegm-dampness constitution group(29 cases).In addition 30 health individuals with moderate TCM constitution and normal BMI had been recruited as the control group.The Th1 and Th2 cell proportion and Th1/Th2 ratio were determined by flow cytometry monoclonal immunofluorescence;The visceral fat area was measured with bioelectrical impedance and abdominal subcutaneous fat thickness was measured with sebum thickness meter;height,waist circumference(WC),waist to hip ratio(WHR),waist to height ratio(WHtR)and other abdominal obesity indexes were observed.The correlation between Th1/Th2 ratio and abdominal obesity related indicators were analyzed.Results:The obesity phlegm-dampness constitution group showed higher Th1 cell proportion and Th1/Th2 ratio than the rest two groups(P<0.05).The obesity phlegm-dampness constitution group and obesity non phlegm-dampness constitution group showed lower Th2 cell proportion than normal group(P<0.05).Correlation analysis showed that Th1/Th2 ratio has a positive correlation with visceral fat area,abdominal subcutaneous fat thickness,WC,WHR and WHtR(r=0.509,r=0.714,r=0.788,r=0.491,r=0.778,P<0.05)in obesity phlegm-dampness constitution group.Conclusions:There is obvious imbalance of Th1/Th2 cell proportion in obesity phlegm-dampness constitution patients,and this change is correlated with the abdominal obesity related indicators.This may be one of the reasons for the higher prevalence of abdominal obesity in phlegm-dampness constitution people.

关 键 词：腹型肥胖 痰湿质 T辅助细胞 Th1/Th2比值 中医体质 

分 类 号：R211[医药卫生—中医学]