2015—2016年上海地区成人门诊腹泻病例中诺如病毒GⅡ型流行特征  

Prevalence of Norovirus GⅡin adult acute gastroenteritis outpatients in Shanghai from 2015 to 2016

作　　者：陈海丽[1] 杨春忆 张万菊[1] 刘祎[1] 田棣[1] 王蔚 易志刚[2] 朱召芹[1] Chen Haili;Yang Chunyi;Zhang Wanju;Liu Yi;Tian Di;Wang Wei;Yi Zhigang;Zhu Zhaoqin(Shanghai Public Health Clinical Center,Shanghai 201508,China;Shanghai Medical College,Fudan University,Shanghai 200032,China)

机构地区：[1]上海市公共卫生临床中心,201508 [2]复旦大学上海医学院,200032

出　　处：《中华实验和临床病毒学杂志》2020年第2期145-150,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：上海市卫生计划和生育委员会科研项目(20164Y0023);十三五科技重大专项(2017ZX10103009,2018ZX10714002-001-005)。

摘　　要：目的了解2015—2016年上海地区成人门诊急性腹泻病例中诺如病毒(norovirus,NoV)GⅡ型的基因分型及分子流行病学特征。方法收集上海地区2015年3月至2016年12月912份成人急性腹泻粪便标本,采用一步法定量逆转录PCR方法检测NoV GⅡ型,并扩增开放阅读框1(open reading frame,ORF1)的聚合酶和ORF2衣壳区域进行测序分型。结果2015年3月至2016年12月912份成人急性腹泻粪便标本NoV GⅡ型阳性检出率为17.76%(162/912),其中2015年NoV GⅡ型阳性率为15.08%(65/431),2016年阳性率为20.17%(97/481),阳性标本中针对ORF1的RdRp区域和ORF2区域5′端均分型的标本为145份,共有10种型别,分别为GⅡ.Pe_GⅡ.4 Sydney 2012(60份)、GⅡ.P17_GⅡ.17(45份)、GⅡ.P16_GⅡ.13(10份)、GⅡ.P12_GⅡ.3(10份)、GⅡ.P7_GⅡ.6(9份)、GⅡ.P21_GⅡ.21(5份)、GⅡ.P16_GⅡ.4 Sydney 2012(2份)、GⅡ.Pe_GⅡ.17(2份)、GⅡ.P2_GⅡ.2(1份)、GⅡ.P7_GⅡ.14(1份)。结论上海地区2015年春季主要流行GⅡ.P17_GⅡ.17,2015/2016年冬春季主要流行GⅡ.Pe_GⅡ.4 Sydney 2012和GⅡ.P17_GⅡ.17,而2016年秋季主要以GⅡ.Pe_GⅡ.4 Sydney 2012流行为主。进行持续的NoV暴发监测对于确定基因型分布的变化趋势和新毒株的发现非常重要。Objective To understand the genotyping and molecular epidemiological characteristics of Norovirus(NoV)GⅡin adult acute gastroenteritis outpatients in Shanghai from 2015 to 2016.Methods A total of 912 stool specimens from adult patients with acute diarrhea from March 2015 to December 2016(431 in 2015 and 481 in 2016)were collected.The Norovirus GⅡtype was detected by one-step quantitative reverse transcription PCR assay and the RdRp region of open reading frame(ORF)1 and 5′end of ORF2 were amplified.The region was sequenced and classified.Results From March 2015 to December 2016,NoV GⅡwere detected in 17.76%(162/912)of the samples,15.08%(65/431)in 2015 and 20.17%(97/481)in 2016.Based on sequence analysis of the RdRp and capsid sequences,145 identified NoV strains were divided into 10 genotypes:GⅡ.Pe_GⅡ.4 Sydney 2012(60),GⅡ.P17_GⅡ.17(45),GⅡ.P16_GⅡ.13(10),GⅡ.P12_GⅡ.3(10),GⅡ.P7_GⅡ.6(9),GⅡ.P21_GⅡ.21(5),GⅡ.P16_GⅡ.4 Sydney 2012(2),GⅡ.Pe_GⅡ.17(2),GⅡ.P2_GⅡ.2(1),and GⅡ.P7_GⅡ.14(1).Conclusions The main epidemic NoV GⅡgenotype in Shanghai was GⅡ.P17_GⅡ.17 in the spring of 2015.GⅡ.Pe_GⅡ.4 Sydney 2012 and GⅡ.P17_GⅡ.17 were identified as predominant genotypes during the winter of 2015 and spring of 2016.The most common genotype was GⅡ.Pe_GⅡ.4 Sydney 2012 in autumn of 2016.Continuous NoV outbreak surveillance is important for identifying changing trends in genotype distribution and emerging new strains.

关 键 词：诺如病毒 GⅡ型 成人腹泻 

分 类 号：R181[医药卫生—流行病学] R51[医药卫生—公共卫生与预防医学]