新型冠状病毒肺炎核酸检测现状及研究进展  

Research progress on nucleic acid detection in COVID-19

作　　者：董召刚[1] 邹明瑾[1] 张义[1] Dong Zhaogang;Zou Mingjin;Zhang Yi(Department of Clinical Laboratory,Qilu Hospital of Shandong University,Shandong Province Key Laboratories of Medicine and Health(Tumor Marker Translational Medicine Laboratory),Jinan 250012,China)

机构地区：[1]山东大学齐鲁医院检验科,山东省医药卫生重点实验室(肿瘤标志物转化医学实验室),济南250012

出　　处：《中华检验医学杂志》2020年第4期386-390,共5页Chinese Journal of Laboratory Medicine

基　　金：山东省自然科学基金面上项目(ZR2017MH044);山东省重点研发计划(2016GSF201124,2016GSF201122);山东大学齐鲁医院住院医师规范化培训创新性研究专项(ZPZX2017A05)。

摘　　要：新型冠状病毒肺炎防控处于关键阶段,核酸检测作为确诊手段,在快速诊断、疗效评估、疫情防控中发挥重要作用。但是由于新型冠状病毒肺炎为突发疫情,核酸检测方法应用于临床的时间短,尚未进行足够的性能验证及临床应用评估,在实际工作中出现了部分与临床诊断不符的假阴性现象。因此,优化当前的检测手段、提高检测灵敏度是亟待解决的问题。本文结合新型冠状病毒最新研究成果及发现,对核酸检测的现状及应用前景进行综述,以期为临床医生及科研工作者提供参考。The prevention and control of novel coronavirus pneumonia caused by 2019 novel coronavirus is at a critical period.Nucleic acid detection,as the definite diagnosis tool,plays an important role in rapid diagnosis,therapeutic efficacy,epidemic prevention and control.However,the disease is outbreak,and the time of nucleic acid detection in clinical application is short.So the insufficient method verification and clinical evaluation has been made."False negative"is observed in clinical practice,and the result of nucleic acid detection is not matched with the clinical diagnosis.Therefore,it is urgent to optimize the current methods and improve detection sensitivity.Based on latest studies of 2019 novel coronavirus,this article reviews the current status and application prospects of nucleic acid detection.Also,this article provides references for clinicians and researchers.

关 键 词：冠状病毒属 肺炎 病毒性 核酸类 临床实验室技术 质量控制 

分 类 号：R5[医药卫生—内科学]