流感肺炎患者外周血中PDCD5与Bcl-2蛋白表达及其与淋巴细胞凋亡的关系  

Expression of PDCD5 and Bcl-2 in peripheral blood of the patients with influenza pneumonia and the potential relationship with lymphocyte apoptosis

作　　者：李明[1] 苏维[2] 马士恒[1] 王涛[3] 程雪娜 Li Ming;Su Wei;Ma Shi-heng;Wang Tao;Cheng Xue-na(Department of Infectious Disease,the Affiliated Hospital of Hebei University,Baoding 071000,China)

机构地区：[1]河北大学附属医院感染疾病科,河北保定071000 [2]河北大学附属医院检验科,河北保定071000 [3]河北大学附属医院结核科,河北保定071000

出　　处：《中国急救医学》2020年第5期399-403,共5页Chinese Journal of Critical Care Medicine

基　　金：河北大学附属医院项目(2015Q008)。

摘　　要：目的研究凋亡相关蛋白PDCD5和Bcl-2在流感肺炎患者外周血中的表达及其与淋巴细胞凋亡的关系.方法ELISA实验检测流感肺炎患者血清中凋亡相关蛋白PDCD5和Bcl-2表达,并分析其相关性;流式细胞仪检测流感肺炎患者外周血淋巴细胞凋亡;Western blot检测流感肺炎患者外周血淋巴细胞中凋亡相关蛋白PDCD5和Bcl-2表达;流式细胞仪分离检测T细胞亚群含量及凋亡变化,分析T细胞亚群凋亡与血清中凋亡相关蛋白PDCD5和Bcl-2表达的相关性.结果PDCD5在流感肺炎患者血清中表达较健康对照者表达显著增加(μg/L:19.77±8.88 vs.1.32±1.02,P<0.05),Bcl-2表达显著降低(μg/L:6.15±2.10 vs.10.25±3.56,P<0.05),且两者表达呈显著负相关(r=-0.386,P<0.05);流感肺炎患者外周血淋巴细胞凋亡率较健康对照者显著增加(%:39.68±12.15 vs.2.54±1.61,P<0.05),且淋巴细胞中PDCD5高表达(0.81±0.16 vs.0.21±0.06,P<0.05),Bcl-2低表达(0.33±0.14 vs.0.53±0.11,P<0.05);流感肺炎患者外周血CD3+T细胞及CD3+CD4+T细胞亚群和CD3+CD8+T细胞亚群的凋亡比例较健康对照者显著增多(21.19±10.67 vs.12.63±7.15、15.49±8.85 vs.10.56±5.14、15.93±7.89 vs.12.38±6.97,P<0.05),其中CD3+CD4+T细胞亚群凋亡更为显著,且与血清中PDCD5表达呈正相关(r=0.335,P<0.05),与Bcl-2表达呈负相关(r=-0.296,P<0.05);流感肺炎患者与健康对照者比较,T细胞亚群中CD3+CD4+T细胞比例下降(27.32±3.14 vs.39.49±4.41,P<0.05),CD3+CD8+T细胞比例增加(40.81±3.89 vs.28.36±2.85,P<0.05),CD4+/CD8+T细胞比值降低(0.67±0.17 vs.1.39±0.16,P<0.05).结论流感肺炎患者外周血中PDCD5高表达,Bcl-2低表达,两者呈显著负相关,T淋巴细胞凋亡可能与PDCD5和Bcl-2表达有关.Objective To investigate the expression of apoptosis-related proteins PDCD5 and Bel-2 in peripheral blood of patients with influenza pneumonia and their relationship with lymphocyte apoptosis.Methods The levels of PDCD5 and Bel-2 in serum of the patients with influenza pneumonia were detected by ELISA and the correlation was analyzed.The apoptosis of peripheral blood lymphocytes in the patients with influenza pneumonia was detected by flow cytometry,the apoptosis-related proteins PDCD5 and Bel-2 in peripheral blood of the patients with influenza pneumonia were detected by Western blot.The content and apoptosis of T cell subsets were detected by flow cytometry,the correlation between apoptosis of T cell subsets and the expression of PDCD5 and Bel-2 in serum was analyzed.Results PDCD5 was highly expressed in serum of the patients with influenza pneumonia(jig/L:19.77±8.88 vs.1.32±1.02,P<0.05)and Bel-2 expression was low(p g/L:6.15±2.10 vs.10.25±3.56,P<0.05),and the expression of PDCD5 was negatively correlated with Bel-2(r=-0.386,P<0.05).The apoptosis rate of peripheral blood lymphocytes in the patients with influenza pneumonia was significantly higher than that in healthy controls(%:39.68±12.15 vs.2.54±1.61,P<0.05).PDCD5 was highly expressed in peripheral blood lymphocytes of the patients with influenza pneumonia(0.81±0.16 vs.0.21±0.06,P<0.05),and the expression of Bel-2 was low(0.33±0.14 vs.0.53±0.11,P<0.05).The percentage of apoptosis in CD3+T lymphocytes,CD3+,C D 4+T lymphocytes and CD3+,CD8+T lymphocytes in peripheral blood of patients was significantly higher than that in healthy controls(21.19±10.67 vs.12.63±7.15,15.49±8.85 vs.10.56±5.14,15.93±7.89 vs.12.38±6.97,P<0.05),especially in CD3+,C D 4+T lymphocytes.There was a positive correlation between the expression of PDCD5 and the apoptosis rate of CD3+,CD4+T lymphocytes(r=0.335,P<0.05),and a negative correlation between the expression of Bel-2 and the apoptosis rate of CD3+,CD4+T lymphocytes(r=—0.296,P<0.05).The percentage of CD3+,CD4+T lymp

关 键 词：程序化细胞死亡因子5(PDCD5) B淋巴细胞瘤-2基因(Bcl-2) 流感肺炎 淋巴细胞 凋亡 

分 类 号：R563.1[医药卫生—呼吸系统]