内皮祖细胞移植对百草枯中毒致急性肺损伤小鼠酸敏感离子通道-1a的影响  

Effect of endothelial progenitor cells transplantation on acid-sensitive ion channel-1a in acute lung injury induced by paraquat poisoning

作　　者：靳妍[1] 刘志[1] 才权 郭亮[1] 杨琛[1] Jin Yan;Liu Zhi;Cai Quan;Guo Liang;Yang Chen(Emergency Department,the First Affiliated Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院急诊科,辽宁沈阳110001

出　　处：《中国急救医学》2020年第5期444-449,共6页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金资助项目(81772053)。

摘　　要：目的探讨内皮祖细胞(EPCs)移植对百草枯(PQ)中毒所致的急性肺损伤(ALI)小鼠的作用机制。方法采用密度梯度离心联合贴壁培养法分离培养小鼠外周血EPC,为后期移植提供供体细胞。应用腹腔注射法建立百草枯所致的急性肺损伤(PQ-ALI)小鼠模型,建模成功后小鼠分为四组:正常对照组、模型组、PQ损伤组、EPCs治疗组(每组9只),移植后48 h处死小鼠并取肺组织,检测肺组织湿干质量(W/D)比值、肿瘤坏死因子-a(TNF-a)含量及肺组织中酸敏感离子通道-l a(ASICla)的表达。结果①正常对照组与模型组比较小鼠肺W/D无明显变化(两者W/D比值分别为3.72、3.67),差异无统计学意义(P>0.05);PQ损伤组小鼠肺组织W/D比值为9.80,与对照组比较差异有统计学意义(P<0.05);尾静脉E PC移植后(EPCs组)可以明显降低小鼠肺组织W/D比值,其值为4.91,与P Q损伤组比较差异有统计学意义(P<0.05)。②正常对照组及模型组中TNF-a分别为226 n g/L和204 n g/L,两组比较差异无统计学意义(P>0.05);PQ损伤组小鼠肺组织中TNF-a为382 ng/L明显增高,与正常对照组比较差异有统计学意义(P<0.05);EPCs治疗组小鼠肺组织TNF-a为276 n g/L呈下降趋势,与P Q损伤组比较差异有统计学意义(P<0.05)。③PQ损伤组和EPCs治疗组小鼠肺组织中ASICU的表达量分别为1.73和1.12,均较正常对照组ASICla的表达量(0.56)明显增多,差异有统计学意义(P<0.05);EPCs治疗组小鼠肺组织中ASICla的表达量低于PQ损伤组(P<0.05);模型组小鼠ASICla的表达量为0.54和治疗对照组小鼠肺组织中ASICla的表达量最少,两组比较差异无统计学意义(P>0.05)。结论PQ-ALI小鼠模型机制可能和ASICla激活有关,EPC移植可改善PQ中毒所致ALI小鼠状态,可能和下调ASIC1a的表达相关,但具体机制尚未清楚。Objective To investigate the mechanism of endothelial progenitor cells(EPCs)transplantation on acute lung injury(ALI)caused by paraquat(PQ)poisoning.Methods Peripheral blood EPCs were isolated and cultured by density gradient centrifugation combined with adherent culture to provide donor cells for later transplantation.PQ-ALI model was established by intraperitoneal injection of PQ.After successful modeling,the mice were divided into four groups:normal control group,model group,PQ injury group and EPCs treatment group,with 9 mice in each group.At the 48th hour after transplantation,the mice were sacrificed and lung tissue was taken for detection of W/D ratio,expression of TNF-ct,and expression of acid-sensitive ion channel-1 a(ASIC la)in lung tissue.Results①There was no significant change in the W/D ratio between the control group and the model group(the W/D ratio was 3.72 and 3.67,respectively),and the difference was not statistically significant(P>0.05).The W/D ratio of PQ injury group was 9.80,and the difference was statistically significant compared with the control group(P<0.05).After EPCs were transplanted into the tail vein,the W/D ratio of lung tissue in mice was significantly reduced,with a value of 4.91,which was statistically significant compared with the PQ injury group(P<0.05).©T h e TNF-a levels in the control group and the model group were 226 ng/L and 204 n g/L,respectively(P>0•05);TNF-O(382 n g/L)in PQ injury group was significantly increased compared with control group,the difference was statistically significant(P<0.05);TNF-a level(276 n g/L)in the lung tissues of mice in the EPCs transplantation group was decreased,and showed statistically significant difference compared with the PQ injury group(P<0.05).③The expression levels of ASIC la in the lung tissues of the PQ injury group and the EPCs transplantation group were 1.73 and 1.12,which increased significantly compared with the control group(0.56,P<0.05).The expression level of ASIC la in the lung tissues of mice in the EPCs transplant

关 键 词：内皮祖细胞(EPCs) 百草枯(PQ) 急性肺损伤(ALI) 酸敏感离子通道-1a(ASIC1a) 

分 类 号：R595.4[医药卫生—内科学]