GARNL1基因多态位点与鸡冠高度的相关性分析  

Association analysis of gene polymorphisms in the GARNL1 gene with chicken comb height

作　　者：沈栩 李健[3] 和俊[3] 谢袖娟[3] 江丹莉 曾华[3] 李秀金 张续勐 欧阳宏佳 潘建秋 刘文俊 付新亮 黄运茂 张细权[3] SHEN Xu;LI Jian;HE Jun;XIE Xiujuan;JIANG Danli;ZENG Hua;LI Xiujin;ZHANG Xumeng;OUYANG Hongjia;PAN Jianqiu;LIU Wenjun;FU Xinliang;HUANG Yunmao;ZHANG Xiquan(College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou 510225, China;College of Animal Science, South China Agricultural University, Guangzhou 510642, China)

机构地区：[1]仲恺农业工程学院动物科技学院,广东广州510225 [2]广东省水禽健康养殖重点实验室,广东广州510225 [3]华南农业大学动物科学学院,广东广州510642

出　　处：《仲恺农业工程学院学报》2020年第2期11-14,共4页Journal of Zhongkai University of Agriculture and Engineering

摘　　要：鸡冠高度是早熟性性状选育的重要间接指标,鉴定与鸡冠高度相关的候选基因对优质鸡生产具有重要的意义.采用PCR-RFLP方法对宁都三黄鸡母系1301个个体进行基因型检测,分析家鸡繁殖性状侯选基因GARNL1多态位点与母鸡鸡冠高度的相关性,结果表明GARNL1基因上多个位点与鸡的冠高性状显著相关,是影响母鸡鸡冠高度选育的候选基因;位点rs14532831和rs15701119与鸡77日龄冠高显著(P<0.05)或极显著(P<0.01)相关;位点rs14532831,rs14532824和rs15701119与鸡84日龄冠高显著(P<0.05)或极显著(P<0.01)相关.基因上的4个位点rs14532831,rs14532779,rs14532824及rs15701119与鸡91日龄冠高显著(P<0.05)或极显著(P<0.01)相关.GARNL1基因的变异对鸡冠高度性状具有遗传效应.Comb height was one of the important indirect selection index in breeding against sexual maturity,it was great significance to identify candidate genes that were highly correlated with comb height for quality chicken production.PCR-RFLP method had been utilized for genotyping in 1301 individuals of NDH female populations,association analysis between the polymorphisms of the candidate genes GARNL1 with comb height was performed.The results showed that multiple loci of GARNL1 gene were significantly associated with comb height,and GARNL1 was a candidate gene for chicken comb height.Highly significantly associated(P<0.01)were found between rs14532831 and comb height at 77 d age,as well as rs15701119 was significantly associated with comb height at 77 d age(P<0.05);rs15701119,rs14532824 and rs14532831 could be used as effective molecular markers for comb height at 84 d age(P<0.01 or P<0.05);Moreover,4 SNPs rs14532824,rs14532831,rs14532779 and rs15701119 were significantly associated with comb height at 91 d age(P<0.01 or P<0.05).The results demonstrated that GARNL1 gene had genetic effect on comb height.

关 键 词：鸡 GARNL1基因 多态位点 鸡冠高度 相关性分析 

分 类 号：J653[艺术—音乐]