UPLC-MS/MS快速测定小鼠血清中10个茋类成分的含量研究  

Simultaneous rapid detection of ten stilbenes in serum of mice by UPLC-MS/MS

作　　者：刘双双 刘青[1,2] 李珮 杨志宏[1] 何春年[1,2] 肖培根[1,2] LIU Shuang-shuang;LIU Qing;LI Pei;YANG Zhi-hong;HE Chun-nian;XIAO Pei-gen(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College y Beijing 100193,China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Mediciney Ministry of Education,Beijing 100193,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]教育部中草药物质基础与资源利用重点实验室,北京100193

出　　处：《中国中药杂志》2020年第9期2180-2185,共6页China Journal of Chinese Materia Medica

基　　金：国家“重大新药创制”科技重大专项(2018ZX09711-001);中国医学科学院医学与健康科技创新工程经费项目(2016-I2M-1-012);北京工商大学植物资源研究与开发北京市重点实验室开放研究基金项目。

摘　　要：茋类成分是一类以1,2-二苯乙烯为骨架结构的天然多酚类化合物,具有结构和活性多样性,但其体内过程研究较少,限制了该类成分的深入研究和开发。该文首次建立了UPLC-MS/MS快速测定血清中10个茋类化合物的方法,并将其应用于牡丹种皮中10个茋类成分在C57小鼠血清中的含量测定。研究以白皮杉醇为内标,血清样品用甲醇沉淀蛋白,在UPLC-MS/MS的负离子模式下分析,该方法经专属性、线性范围、精密度、准确度、提取回收率和基质效应、稳定性等考察确证可靠适用。持续每天灌胃给予C57小鼠800 mg·kg^(-1)牡丹种皮提取物8周后,收集小鼠血清检测。结果表明,牡丹种皮中suffruticosol A,suffruticosol B,suffruticosol C,trans-ε-viniferin,cis-gnetin H,trans-suffruticosol D和trans-gnetin H可在血清样品中检测到,并且以suffruticosol A浓度最高。该方法灵敏快捷、精密准确、专属稳定,可用于血清中10个茋类化合物的同时定量分析,为阐明茋类成分的体内过程和牡丹种皮中该类成分的药效机制奠定基础。Stilbenes is a class of natural polyphenols with 1,2-diphenylethylene as the skeleton structure which have structural and active diversity.However,there are fewer studies on their metabolic process,which limits the in-depth research and development of such components.An UPLC-MS/MS method simultaneously determining contents of ten stilbenes was firstly established in this study and applied to study the ten stilbenes of peony seed coats in the serum of C57 mice.Piceatannol was the internal standard,and methanol was used for protein precipitation,UPLC-MS/MS with negative ion mode was used for analysis,and the method was validated.The serum samples were collected and detected after mice being oral administered with 800 mg·kg-1 peony seed coat extracts for 8 weeks.The results showed that suffruticosol A,suffruticosol B,suffruticosol C,trans-ε-viniferin,cis-gnetin H,trans-suffruticosol D and trans-gnetin H were detected in serum samples,and the highest is suffruticosol A.The method is simple and quick with high specificity and sensitivity,and it is suitable for quantitative determination of ten stilbenes in the serum of mice.

关 键 词：茋类化合物 牡丹种皮 方法学验证 超高效液相色谱-质谱联用法 含量测定 

分 类 号：R285.5[医药卫生—中药学]