紫铆素通过免疫抑制功能改善莫诺苯宗诱导白癜风小鼠毛发脱色的研究  

Improvement of butin on decolorization in vitiligo mice induced by monobenzone by inhibiting immune function

作　　者：黄海艳[1] 邹彦芬[1] 刘小明 李云会 张杰[1] HUANG Hai-yan;ZOU Yan-fang;LIU Xiao-ming;LI Yun-hui;ZHANG Jie(Department of Dermatology,Peking University Shenzhen Hospital,Shenzhen 518036,China)

机构地区：[1]北京大学深圳医院皮肤科,广东深圳518036

出　　处：《现代药物与临床》2020年第5期825-830,共6页Drugs & Clinic

基　　金：国家自然科学基金青年科学基金资助项目(81803138、81502727)。

摘　　要：目的探讨紫铆素通过免疫抑制功能对莫诺苯宗诱导白癜风小鼠毛发脱色的改善作用。方法 40%莫诺苯宗乳膏诱导小鼠脱色构建白癜风模型,48只C57BL/6小鼠按体质量随机分对照组、模型组、他克莫司组和紫铆素组,每组12只。模型组涂抹40%莫诺苯宗乳膏50 mg,1次/d,他克莫司组在模型组的基础上7 h后涂抹0.1%他克莫司乳膏30 mg,1次/d;紫铆素组在模型组的基础上7 h后0.1%紫铆素乳膏10 mg,1次/d,连续用药50 d。观察并记录小鼠的毛发脱色情况;分离小鼠脾脏淋巴细胞,CFSE法检测T淋巴细胞和B淋巴细胞增殖情况;采用ELISA法检测各组小鼠血清中IgM、IgA和IgG水平;3H-TdR标记法检测IL-2分泌活性;流式细胞仪检测表面抗原CD19活性。结果与模型组比较,紫铆素能显著改善白癜风小鼠毛发脱色现象,显著降低脱色发生率、显著减小脱色面积,差异有统计学意义(P<0.05);与模型组比较,紫铆素能显著抑制淋巴细胞增殖活性,差异有统计学意义(P<0.05);与模型组比较,紫铆素能显著降低小鼠血清中IgM、IgG、IgA水平(P<0.05),上调IL-2分泌活性和增强表面抗原CD19活性(P<0.05)。结论紫铆素可以改善白癜风小鼠毛发脱色的现象,其作用机制可能是通过抑制小鼠自身免疫反应。Objective To investigate the improvement of butin on decolorization in vitiligo mice induced by monobenzone by inhibiting immune function.Methods 40%Monorubicin cream was used to induce decolorization of mice to construct vitiligo model.48 C57BL/6 mice were randomly divided into control group,model group,tacrolimus group,and butin group according to the weight.Rats in the model group were applied with 40%Monobenzone Cream 50 mg,once daily.Rats in the tacrolimus group were applied with 0.1%Tacrolimus Cream 30 mg on the basis of model group after 7 h,once daily.Rats in the butin group were applied with 0.1%Butin Cream 10 mg on the basis of model group after 7 h,once daily.They were treated for 50 d.The hair discoloration of mice were observed and recorded.The spleen lymphocytes were isolated,and the proliferation of T lymphocytes and B lymphocytes were detected by CFSE assay.The levels of IgM,IgA and IgG in serum were detected by ELISA method.The secretion activity of IL-2 was detected by 3 H-TdR labeling method,and the activity of CD19 was detected by flow cytometry.Results Compared with the model group,butin can significantly improve hair decolorization in mice with vitiligo,reduce the incidence and reduce the area of decolorization,with significantly difference between two groups(P<0.05).Compared with the model group,butin can significantly inhibit lymphocyte proliferation,with significantly difference between two groups(P<0.05).Compared with the model group,butin can significantly decrease IgM,IgG,and IgA levels in serum(P<0.05),and increase IL-2 secretion activity and surface antigen CD19 activity in serum(P<0.05).Conclusion Butin can improve the hair discoloration in mice with vitiligo,and its mechanism may be through inhibiting the autoimmune activity of mice.

关 键 词：紫铆素 他克莫司 白癜风 免疫反应 

分 类 号：R966[医药卫生—药理学] R285.5[医药卫生—药学]