Ogura CMS育性恢复基因orf687在萝卜中的分布  

Distribution of orf687,a Fertility Restorer Gene for Ogura CMS in Radish

作　　者：张丽[1] 王庆彪[1] 王艳萍 ZHANG Li;WANG Qingbiao;WANG Yanping(Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture and Rural Affairs,P.R.China,Beijing Key Laboratory of Vegetable Germplasm Improvementy Beijing 100097,China)

机构地区：[1]北京市农林科学院蔬菜研究中心,农业农村部华北地区园艺作物生物学与种质创制重点实验室,蔬菜种质改良北京市重点实验室,北京100097

出　　处：《园艺学报》2020年第5期864-874,共11页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31872099);北京市科技新星计划项目(XX2018086)。

摘　　要：以129个萝卜品种(包括5个栽培种和4个野生种)为试材,研究了Ogura CMS细胞核育性恢复基因orf687(Rfo)的分布。对orf687基因扩增的结果表明,除油用萝卜外,orf687目的片段广泛分布于东亚大长萝卜(83份,89.16%)、欧洲小萝卜(28份,85.71%)、黑萝卜(6份,100%)、荚用萝卜(1份,100%)和野生萝卜(10份,70%)中。东亚大长萝卜中的红萝卜品种95.45%含有orf687,高于白萝卜和绿萝卜。进一步利用PCR-RFLP对orf687的基因型进行鉴定发现,没有恢复功能的基因型(rfrf)在野生萝卜中所占比率最高,为57.14%,其次是东亚大长萝卜(47.13%)和欧洲小萝卜(37.50%)。在东亚大长萝卜中,红萝卜品种具有rfrf基因型的比率为76.19%,高于白萝卜(41.67%)和绿萝卜(23.53%)。在利用萝卜细胞质雄性不育材料育种的过程中,rfrf基因型的材料没有育性恢复功能,可作为保持系,用于雄性不育系的转育。上述结果可为萝卜雄性不育系的选育和利用提供指导。The distribution of orf687(Rfo),a fertility restorer gene of Ogura CMS,was investigated in 129 radish cultivars,including 5 cultivated species and 4 wild species. Our results showed that orf687 was widely distributed in East Asian big radish(89.16%),European small radish(85.71%),black radish(100%),pod radish(100%)and wild radish(70%). Among East Asian big radish,95.45% of the red-skinned radish contained orf687,which was higher than that of white radish and green radish. In addition,genotype identification of orf687 by PCR-RFLP showed that the ratio of double recessive genotype(rfrf)without restoring ability was the highest in wild radish(57.14%),followed by East Asia big radish(47.13%)and European small radish(37.50%). Moreover,in East Asian big radish,the ratio of red-skinned varieties with rfrf genotype was 76.19%,higher than that of white radish(41.67%)and green radish(23.53%). The results clarified the Rfo genotypes of different radish varieties,and will provide guidance for the breeding and utilization of male sterile lines.

关 键 词：萝卜 Ogura CMS 育性恢复基因 分布 

分 类 号：S631.1[农业科学—蔬菜学]