结球甘蓝抽薹性遗传规律和QTL定位分析  

Genetic and QTL Mapping Analysis of Bolting Time in Cabbage(Brassica oleracea)

作　　者：王五宏[1] 汪精磊 李必元[1] 魏庆镇 胡天华[1] 胡海娇[1] 包崇来[1] WANG Wuhong;WANG Jinglei;LI Biyuan;WEI Qingzhen;HU Tianhua;HU Haijiao;BAO Chonglai(Institute of Vegetables,Zhejiang Academy of Agricultural Sciences*Hangzhou 310021,China)

机构地区：[1]浙江省农业科学院蔬菜研究所,杭州310021

出　　处：《园艺学报》2020年第5期974-982,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31372058);浙江省重大科技专项(2012C12903-6)。

摘　　要：以早抽薹‘S-1’与晚抽薹‘G-1’为亲本,构建F1、BC1、BC2和F2群体,采用植物数量性状主基因+多基因混合遗传模型分析法对结球甘蓝抽薹性状进行遗传分析,并采用SLAF-BSA方法对抽薹时间进行QTL定位分析。结果显示,结球甘蓝抽薹性状是由2对加性-显性-上位性主基因+加性-显性多基因遗传控制;主基因+多基因平均遗传率是93.41%。共检测到2个QTL,分别为2号染色体上的2.31~3.09 Mb和33.57~34.40 Mb,总长度为1.61 Mb。We constructed the populations of F1,BC1,BC2,and F2 by using cabbage lines ‘S-1’(early flower)and ‘G-1’(late flower)as parents and adopted the plant quantitative trait major gene + polygenic mixed genetic model method to analysis the genetic model of bolting time of cabbage. Besides,we used SLAF-BSA technology to mapping the bolting time QTLs. The results showed that the bolting time fitted the model of two additive-dominant-epistatic major genes and additive-dominance polygenes. The average heritability of major genes plus polygenes was 93.41%,which indicated that bolting trait of cabbage was mainly affected by major gene inheritance. Moreover,two QTLs were detected,which located on chromosome C02 2.31–3.09 Mb and 33.57–34.40 Mb,respectively,with a total length of 1.61 Mb.

关 键 词：甘蓝 抽薹 遗传分析 QTL定位 

分 类 号：S635.1[农业科学—蔬菜学]