糖尿病模型兔眼结膜损伤的病理机制研究  

Pathological Mechanism of Conjunctival Injury in Diabetic Model Rabbits

作　　者：葛健[1] 朱彩红[1] 吴彦霖[1] 吴永杰[2] GE Jian;ZHU Caihong;WU Yanlin;WU Yongjie(Ophthalmology,RuiJin Hospital,Shanghai J~iao Tbng University Medical School,Shanghai 200025,China;RuiJin Hospital,Shanghai Jiao To^g University Medical School,Shanghai Institute of Hypertension,Shanghai Key Laboratory of Hypertension,Shanghai 200025,China)

机构地区：[1]上海交通大学医学院附属瑞金医院眼科,上海200025 [2]上海交通大学医学院附属瑞金医院,上海市高血压研究所,上海市高血压重点实验室,上海200025

出　　处：《实验动物与比较医学》2020年第2期136-140,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的通过检测高脂高糖饲料饲喂加链佐脲菌素诱发的糖尿病兔结膜组织细胞的基质金属蛋白酶(MMPs)和基质金属蛋白酶组织抑制剂(TIMPs),分析二者与眼结膜病变间关系。方法选取日本大耳白兔20只,随机分成2组。对照组喂饲普通实验兔饲料;实验组饲喂高脂高糖饲料8周后,耳缘静脉注射链佐脲菌素制备糖尿病模型。在完成模型制作后2周,2组兔经全身麻醉后分别选取双眼的球结膜组织(2 mm×3 mm)各1片。右眼球结膜用于常规病理观察;左眼球结膜用于MMP-1、MMP-3、MMP-9测定,以及TIMP-1、TIMP-3的检测。结果实验组中MMP-1、MMP-3和MMP-9表达高于对照组(P<0.05)。实验组中TIMP-1表达略高于对照组(P>0.05),但TIMP-3表达明显低于对照组(P<0.05)。实验组兔眼结膜上皮排列整齐,上皮下组织明显减少,结膜组织疏松,可见萎缩的筋膜组织,结膜固有层细胞松散,且细胞间炎性细胞增多。结论糖尿病兔结膜组织MMPs和TIMPs的平衡打破,使结膜组织中产生弹力纤维减少,结膜下组织疏松;筋膜萎缩的病理改变,使糖尿病兔结膜出现松弛,造成兔眼表微环境产生改变。Objective To analyze matrix metalloproteinases(MMPs)and tissue inhibitor of matrix metalloproteinases(TMPs)in the conjunctival tissue cells of diabetic rabbits,and to analyze the relationship between MMPs and TMPs.Methods Twenty Japanese white rabbits were randomly divided into 2 groups,the rabbits in control group were fed with the normal experimental rabbit diet,while the diabetic experiment group was feed with high fat and high sugar diet for 8 weeks,and then injected with streptozourease by auricular vein for establishing diabetic model rabbits.Two weeks after the model was made,two groups of rabbits were anesthetized with general anesthesia,and one piece of 2 mm×3 mm bulbar conjunctiva tissue of each eye was sampled respectively.The right conjunctiva was used for pathological observation,and left eye conjunctiva was used for detecting the MMP-1,MMP-3,MMP-9 and TMP-1,TMP-3.Results The expressions of MMP-1,MMP-3,MMP-9 in the experiment group were high compared with those of the control group(P<0.05),and the expression of TMP-1 in the experimental group was slightly higher compared with that of the control group(P<0.05),but TMP-3 was significantly lower(P>0.05).In the diabetic experimental group rabbits,the conjunctival epithelium was arranged in order,subepithelial tissue was obviously reduced,conjunctival tissue was loose,atrophic fascia tissue was observed,the cells of the lamina propria of conjunctiva were loose,and the inflammatory cells between the cells were increased.Discussion Break down of the balance between MMPs and TMPs expression may produce elastic fibers in bulbar conjunctiva,and pathological changes of hypoconjunctiva tissue,the fascia atrophy changed the microenvironment of diabetic rabbit.

关 键 词：基质金属蛋白酶 基质金属蛋白酶组织抑制剂 糖尿病模型 兔 结膜损伤 

分 类 号：Q95-33[生物学—动物学]