佛波酯诱导THP-1分化为M0型巨噬细胞的条件优化  

Optimization of PMA Stimulation Conditions for Inducing the Differentiation of THP-1 into M0 Macrophages

作　　者：王文芮 李海燕 常江 WANG Wenrui;LI Haiyan;CHANG Jiang(School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai,200030;Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai,200050)

机构地区：[1]上海交通大学生物医学工程学院,上海200030 [2]中国科学院上海硅酸盐研究所,上海200050

出　　处：《生物医学工程学进展》2020年第2期74-78,97,共6页Progress in Biomedical Engineering

摘　　要：目的优化诱导THP-1细胞分化为M0型巨噬细胞所需的佛波酯(PMA)浓度和刺激时间。为研究生物活性材料对巨噬细胞表型和功能的调控作用奠定实验基础。方法分析不同PMA浓度与刺激时间下THP-1细胞的形态、贴壁程度、M0型巨噬细胞表面标志物以及巨噬细胞M1和M2表型相关基因的表达。结果在25~100 ng/mL PMA浓度范围和24~72 h刺激时间范围内,当PMA浓度为75 ng/mL,刺激时间为72 h时,既能有效诱导THP-1细胞分化为M0型巨噬细胞,又能对THP-1细胞中M1型巨噬细胞标志基因IL-6、IL-1β、CD86和M2型巨噬细胞标志基因IL-10、CD163、CD206产生相对较低的上调作用。结论优化的诱导THP-1细胞分化为M0型巨噬细胞所需的PMA浓度和刺激时间为75 ng/mL和72 h。Objective To optimize the PMA concentration and stimulation time required for inducing the differentiation of THP-1 cells into M0 macrophages.Methods Analyze the morphology,the adherence,the expression of M0 macrophages surface marker and the gene expression of M1 and M2 macrophages markers of THP-1 cells stimulated with PMA at different concentrations for different times.Results In the range of 25~100 ng/mL PMA and 24~72 h stimulation,using 75 ng/mL PMA to stimulate THP-1 cells for 72 h could effectively induce the differentiation of THP-1 cells into M0 macrophages and caused relatively less up-regulation of M1 and M2 macrophages marker gene expression in THP-1 cells.Conclusion The optimal PMA concentration and stimulation time for inducing the differentiation of THP-1cells into M0 macrophages was 75 ng/mL and 72 h.

关 键 词：THP-1 巨噬细胞 佛波酯 

分 类 号：R319[医药卫生—基础医学]