低浓度苯职业接触工人尿中苯巯基尿酸与8-羟基脱氧鸟苷的分布特征  

Distribution of S-phenylmercapturic acid and 8-hydroxy-2’-deoxyguanosine in urine of workers exposed to low-concentration benzene

作　　者：王坤 王彤 周燕华 浦跃朴[1] 张娟[1] WANG Kun;WANG Tong;ZHOU Yan-hua;PU Yue-pu;ZHANG Juan(School of Public Health,Southeast University,Nanjing,Jiangsu 210009,China)

机构地区：[1]东南大学公共卫生学院,江苏南京210009

出　　处：《环境与职业医学》2020年第5期413-420,共8页Journal of Environmental and Occupational Medicine

摘　　要：[背景]苯是公认的致癌物。随着生产工艺的发展和劳动防护设施的改善,许多作业场所的空气苯浓度已经远低于职业接触限值,但研究发现,低水平苯接触依然会对职业人群健康造成影响。[目的]研究低浓度苯职业接触工人尿液中苯巯基尿酸(S-PMA)与8-羟基脱氧鸟苷(8-OHdG)的分布特征。[方法]采用横断面调查方法,以206名扬州某涉苯汽车制造工厂2018—2019年间参加职业健康检查的职工作为研究对象;其中120名汽车喷漆车间作业工人作为接触组,86名非车间工作的文职管理人员作为对照组。收集研究对象的职业接触与健康检查资料及外周血和尿液样本。对血液样本进行血常规检查;通过高分辨四级杆飞行时间质谱仪检测尿中S-PMA含量,并以尿肌酐校正后的S-PMA测量值作为内暴露指标;采用比色法检测血清中脂质过氧化物丙二醛(MDA)、尿液中8-OHdG水平。校正性别、年龄、体重指数、吸烟及饮酒情况后,分析苯接触对工人外周血象和氧化损伤的影响,并分析S-PMA与8-OHdG的相关性。以8-OHdG水平作为应变量,S-PMA水平及可能影响8-OHdG水平的因素为自变量,进行多元线性逐步回归分析。[结果]该厂产苯环节主要发生在调漆、喷漆过程中。虽各接苯岗位苯接触浓度没有超过职业接触限值,但接触组工人尿液中S-PMA的中位数为12.21μg·g^-1(以Cr计,后同),高于对照组(4.80μg·g^-1,P <0.001);接触组MDA、8-OHdG的中位数分别为11.19μmol·L^-1、169.18 ng·g^-1,均高于对照组(MDA、8-OHdG的中位数分别为9.08μmol·L^-1、91.43 ng·g^-1,P <0.001);接触组红细胞异常率为10.0%,高于对照组(2.3%,P <0.05)。调整吸烟因素后,在吸烟与不吸烟人群中,接触组S-PMA和8-OHdG水平均高于对照组(P <0.05);仅在不吸烟人群中,接触组MDA水平高于对照组(P <0.05);血常规指标异常率在吸烟与不吸烟人群中差异无统计学意义。Spearman相关分析结果显示,[Background]Benzene is a recognized carcinogen.With the development of production technology and the improvement of labor protection facilities,the concentrations of benzene in the air of many workplaces have been far lower than the national occupational exposure limit.However,a large number of studies have found that low-concentration benzene still has an impact on the health of occupational populations.[Objective]This study is conducted to characterize the distributions of S-phenylmercapturic acid(S-PMA)and 8-hydroxy-2’-deoxyguanosine(8-OHdG)in urine of workers exposed to low-concentration benzene.[Methods]A cross-sectional survey was carried out to enroll 206 employees who took part in occupational health examination from a benzene-related automobile factory in Yangzhou from 2018 to 2019,including 120 workers in the automobile painting workstation as the exposure group and 86 office workers as the control group.The occupational exposure data,health examination data,and peripheral blood and urine samples were collected from the subjects.The blood samples were examined for routine blood test indicators.The urine samples were examined for the level of S-PMA by high-resolution quadrupole coupled with time-of-flight mass spectrometer,and the S-PMA level corrected by urine creatinine was taken as an internal exposure index.The contents of lipid peroxide malondialdehyde(MDA)in serum and 8-OHdG in urine were detected by colorimetry as indices of oxidative damage of lipid and DNA.After adjusting for sex,age,body mass index,smoking,and drinking,the effects of benzene exposure on peripheral blood and oxidative damage in workers and the correlations of S-PMA with 8-OHdG were analyzed.Multiple linear stepwise regression analysis was conducted with 8-OHdG as a dependent variable and S-PMA and possible influencing factors of 8-OHdG as independent variables.[Results]Benzene release mainly occurred in the processes of paint mixing and painting in the automobile factory.Although the benzene concentration of each benzene expos

关 键 词：苯 苯巯基尿酸 氧化损伤 丙二醛 8-羟基脱氧鸟苷 

分 类 号：R134[医药卫生—劳动卫生]