林丹对大鼠生精细胞活化型半胱天冬酶-3表达及血清黄体生成素、睾酮浓度的影响  

Effects of lindane on cleaved caspase-3 expression in spermatogenic cells and serum luteinizing hormone and testosterone concentrations in rats

作　　者：曹红亭 孙祖越[3] 周莉[3] 罗玉珍 周健 汪岭 陈健茂 李晓玉 宋琦如 CAO Hong-ting;SUN Zu-yue;ZHOU Li;LUO Yu-zhen;ZHOU Jian;WANG Ling;CHEN Jian-mao;LI Xiao-yu;SONG Qi-ru(School of Public Health and Management,Ningxia Medical University,Yinchuan,Ningxia 750004,China;School of Animal Experimental Center,Ningxia Medical University,Yinchuan,Ningxia 750004,China;Laboratory of Pharmacology and Toxicology,Shanghai Institute of Planned Parenthood,Shanghai 200032,China)

机构地区：[1]宁夏医科大学公共卫生与管理学院,宁夏银川750004 [2]宁夏医科大学实验动物中心,宁夏银川750004 [3]上海市计划生育科学研究所药理毒理学研究室,上海200032

出　　处：《环境与职业医学》2020年第5期508-513,共6页Journal of Environmental and Occupational Medicine

基　　金：宁夏卫生计生委重点科研课题(2018-NW-004)。

摘　　要：[背景]林丹是有机氯农药六六六的γ异构体,对雄性生殖系统有损害作用。[目的]研究林丹亚慢性染毒对雄性大鼠睾丸组织活化型半胱天冬酶-3(cleaved caspase-3)表达及血清黄体生成素、睾酮浓度的影响,探讨林丹致雄性生殖系统损伤的机制。[方法 ] 40只雄性SD大鼠被随机分为5组,分别为低、中、高剂量(3、6、13 mg·kg^-1)林丹染毒组,溶剂对照组(10 mg·kg^-1玉米油),阳性对照组(100μg·kg^-1雌二醇)。每天灌胃1次,连续8周,染毒末24 h称重,禁食一晚经腹腔注射10%水合氯醛溶液麻醉后解剖取材,测定大鼠睾丸生精细胞cleaved caspase-3蛋白表达情况及血清黄体生成素、睾酮浓度。[结果 ]与溶剂对照组相比:各染毒组大鼠体重差异均无统计学意义(P> 0.05);13 mg·kg^-1组大鼠精子密度降低(P <0.05);6、13 mg·kg^-1组大鼠精子存活率下降(P <0.05),睾丸生精细胞cleaved caspase-3蛋白表达升高(P <0.05);13 mg·kg^-1组大鼠血清黄体生成素浓度升高(P <0.05),睾酮浓度降低(P <0.05)。与阳性对照组相比:各染毒组大鼠体重及精子密度差异均无统计学意义(P> 0.05);3、6 mg·kg^-1组大鼠精子存活率升高(P <0.05),睾丸生精细胞cleaved caspase-3蛋白表达降低(P <0.05),血清黄体生成素浓度降低(P <0.05);各染毒组大鼠血清睾酮浓度升高(P <0.05)。病理结果显示:各林丹染毒组大鼠睾丸出现不同程度的损害状态,表现为曲细精管管腔间隙增大,生精细胞排列紊乱,精子数量减少。阳性对照组亦表现为管腔增大,精子数量减少,并可在管腔内发现脱落的生精细胞。[结论 ]林丹可能通过上调睾丸组织cleaved caspase-3蛋白表达来调控生精细胞凋亡,引发血清睾酮水平下降,继而导致大鼠精子生成减少,且在此实验条件下,林丹引起大鼠内分泌干扰作用较雌二醇弱。[Background]Lindane is the gamma isomer of organochlorine pesticide hexachlorocyclohexane and can damage male reproductive system.[Objective]This experiment investigates the effects of sub-chronic exposure to lindane on cleaved caspase-3 expression in testicular tissues and serum luteinizing hormone and testosterone concentrations in male rats,aiming to explore the mechanism of lindane-induced male reproductive damage.[Methods]Forty male SD rats were randomly divided into five groups:solvent control group(10mg·kg-1 corn oil),positive control group(100μg·kg-1 estradiol),and 3,6,and 13mg·kg-1 lindane groups.The rats were treated with the designed dosages by gavage once a day for continuously eight weeks.After 24 h of last exposure,the rats were weighed,and after an overnight fasting,the rats were anesthetized with 10%chloral hydrate and dissected to determine the cleaved caspase-3 protein expression in spermatogenic cells and serum luteinizing hormone and testosterone concentrations.[Results]Compared with the solvent control group,there was no significant difference in body weight of rats in the exposure groups(P>0.05);the sperm density of rats in the 13mg·kg-1 group decreased(P<0.05);the sperm survival rate decreased and the cleaved caspase-3 protein expression level in spermatogenic cells were upregulated in the 6mg·kg-1 and 13mg·kg-1 groups(P<0.05);the serum luteinizing hormone concentration increased and the serum testosterone concentration decreased in the 13 mg·kg-1 group(P<0.05).Compared with the positive control group,there were no significant differences in body weight and sperm density in the exposure groups(P>0.05);the sperm survival rate increased,the protein expression level of cleaved caspase-3 in spermatogenic cells decreased,and the serum luteinizing hormone concentration decreased in the 3 mg·kg-1 and 6mg·kg-1 groups(P<0.05);the serum testosterone concentration in each exposure group increased(P<0.05).The testicular pathological observations showed that the rats in each lindane-treated gr

关 键 词：林丹 凋亡 黄体生成素 睾酮 活化型半胱天冬酶-3 

分 类 号：R114[医药卫生—卫生毒理学]